ArticleBiophysical journal2016
Membrane Shape Instability Induced by Protein Crowding.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed.
- VAMP8 function reveals tight linkage between endocytic recycling and endocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Macromolecular Crowding in Mitochondria.Sub-cellular biochemistry · 2025Review
- The Functionality of Membrane-Inserting Proteins and Peptides: Curvature Sensing, Generation, and Pore Formation.The Journal of membrane biology · 2023Review
- Antibody binding reports spatial heterogeneities in cell membrane organization.Nature communications · 2023Article
- Strength in numbers: effect of protein crowding on the shape of cell membranes.Biochemical Society transactions · 2022Review
- Crowding-induced membrane remodeling: Interplay of membrane tension, polymer density, architecture.Biophysical journal · 2022Article
- The endophilin curvature-sensitive motif requires electrostatic guidance to recycle synaptic vesicles in vivo.Developmental cell · 2022Article
- Observed steric crowding at modest coverage requires a particular membrane-binding scheme or a complementary mechanism.Biophysical journal · 2022Article
- FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria.PLoS biology · 2021Article
- Cellular Membranes, a Versatile Adaptive Composite Material.Frontiers in cell and developmental biology · 2020Review
- Clustering and dynamics of crowded proteins near membranes and their influence on membrane bending.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Simple differences in the protein-membrane attachment mechanism have functional consequences for surface mechanics.The Journal of chemical physics · 2019Article
- Kinetics of Histidine-Tagged Protein Association to Nickel-Decorated Liposome Surfaces.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration.ACS synthetic biology · 2019Article
- Lipids or Proteins: Who Is Leading the Dance at Membrane Contact Sites?Frontiers in plant science · 2019Review
- Modeling Membrane Curvature Generation due to Membrane⁻Protein Interactions.Biomolecules · 2018Review
- Ectopic Neo-Formed Intracellular Membranes inBiomolecules · 2018Review
- Simulations of N-BAR Protein Interactions with Membranes.Journal of physics D: Applied physics · 2018Article
- Structure Versus Stochasticity-The Role of Molecular Crowding and Intrinsic Disorder in Membrane Fission.Journal of molecular biology · 2018Review
- The 2018 biomembrane curvature and remodeling roadmap.Journal of physics D: Applied physics · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Peripheral proteins can bend membranes through several different mechanisms, including scaffolding, wedging, oligomerization, and crowding. The crowding effect in particular has received considerable attention recently, in part because it is a colligative mechanism-implying that it could, in principle, be explored by any peripheral protein. Here we sought to clarify to what extent this mechanism is exploited by endocytic accessory proteins. We quantitatively investigate membrane curvature generation by means of a GUV shape stability assay. We found that the amount of crowding required to induce membrane curvature is correlated with membrane tension. Importantly, we also revealed that at the same membrane tension, the crowding mechanism requires far higher protein coverage to induce curvature changes compared to those observed for the endophilin BAR domain, serving here as an example of an endocytic accessory protein. Our results are important for the design of membrane-targeted biosensors as well as the understanding of mechanisms of biological membrane shaping.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.