ArticleStructure (London, England : 1993)2016
Antibody-Based Affinity Cryoelectron Microscopy at 2.6-Å Resolution.
Article in Structure (London, England : 1993), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- The graphene-based affinity cryo-EM grid for the endogenous protein structure determination.bioRxiv : the preprint server for biology · 2025Article
- Open architecture of archaea MCM and dsDNA complexes resolved using monodispersed streptavidin affinity CryoEM.Nature communications · 2024Article
- An immobilized antibody-based affinity grid strategy for on-grid purification of target proteins enables high-resolution cryo-EM.Communications biology · 2024Article
- Conventional Electron Microscopy, Cryogenic Electron Microscopy, and Cryogenic Electron Tomography of Viruses.Sub-cellular biochemistry · 2024Review
- A 3D-printed flow-cell for on-grid purification of electron microscopy samples directly from lysate.Journal of structural biology · 2023Article
- Article
- Developing Graphene Grids for Cryoelectron Microscopy.Frontiers in molecular biosciences · 2022Review
- High resolution single particle Cryo-EM refinement using JSPR.Progress in biophysics and molecular biology · 2021Review
- From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Human parainfluenza virus fusion complex glycoproteins imaged in action on authentic viral surfaces.PLoS pathogens · 2020Article
- Structural Biology in the Multi-Omics Era.Journal of chemical information and modeling · 2020Article
- Structural and biophysical characterizations of HIV-1 matrix trimer binding to lipid nanodiscs shed light on virus assembly.The Journal of biological chemistry · 2019Article
- Structural Variability in the RLR-MAVS Pathway and Sensitive Detection of Viral RNAs.Medicinal chemistry (Shariqah (United Arab Emirates)) · 2019Review
- Routine determination of ice thickness for cryo-EM grids.Journal of structural biology · 2018Article
- Biological Applications at the Cutting Edge of Cryo-Electron Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2018Review
- Cryo-EM in drug discovery: achievements, limitations and prospects.Nature reviews. Drug discovery · 2018Review
- Approaches to altering particle distributions in cryo-electron microscopy sample preparation.Acta crystallographica. Section D, Structural biology · 2018Review
- Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions.Nature communications · 2018Article
- Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Cryo-EM: beyond the microscope.Current opinion in structural biology · 2017Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The affinity cryoelectron microscopy (cryo-EM) approach has been explored in recent years to simplify and/or improve the sample preparation for cryo-EM, which can bring previously challenging specimens such as those of low abundance and/or unpurified ones within reach of the cryo-EM technique. Despite the demonstrated successes for solving structures to low to intermediate resolutions, the lack of near-atomic structures using this approach has led to a common perception of affinity cryo-EM as a niche technique incapable of reaching high resolutions. Here, we report a ∼2.6-Å structure solved using the antibody-based affinity grid approach with low-concentration Tulane virus purified from a low-yield cell-culture system that has been challenging to standard cryo-EM grid preparation. Quantitative analyses of the structure indicate data and reconstruction quality comparable with the conventional grid preparation method using samples at high concentration.
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