ArticleJournal of visualized experiments : JoVE2016
Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Sex differences in arterial stiffness in a rat model of type 1 diabetes.Frontiers in physiology · 2026Article
- Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis.Science advances · 2024Article
- Loss-of-function G6PD variant moderated high-fat diet-induced obesity, adipocyte hypertrophy, and fatty liver in male rats.The Journal of biological chemistry · 2024Article
- A method for analyzing AFM force mapping data obtained from soft tissue cryosections.Journal of biomechanics · 2024Article
- Stiffness Measurement of Retinal Capillaries and Subendothelial Matrix using Atomic Force Microscopy.bioRxiv : the preprint server for biology · 2024Article
- Review
- Article
- Survivin as a mediator of stiffness-induced cell cycle progression and proliferation of vascular smooth muscle cells.APL bioengineering · 2023Article
- Connecting Aortic Stiffness to Vascular Contraction: Does Sex Matter?International journal of molecular sciences · 2022Article
- Using Polyacrylamide Hydrogels to Model Physiological Aortic Stiffness Reveals that Microtubules Are Critical Regulators of Isolated Smooth Muscle Cell Morphology and Contractility.Frontiers in pharmacology · 2022Article
- A machine learning pipeline revealing heterogeneous responses to drug perturbations on vascular smooth muscle cell spheroid morphology and formation.Scientific reports · 2021Article
- Atomic Force Microscope Nanoindentation Analysis of Diffuse Astrocytic Tumor Elasticity: Relation with Tumor Histopathology.Cancers · 2021Article
- Review
- Surface engineered polymersomes for enhanced modulation of dendritic cells during cardiovascular immunotherapy.Advanced functional materials · 2019Article
- Predicting Ligand-Free Cell Attachment on Next-Generation Cellulose-Chitosan Hydrogels.ACS omega · 2018Article
- Modulating cell response on cellulose surfaces; tunable attachment and scaffold mechanics.Cellulose (London, England) · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Arterial stiffening is a significant risk factor and biomarker for cardiovascular disease and a hallmark of aging. Atomic force microscopy (AFM) is a versatile analytical tool for characterizing viscoelastic mechanical properties for a variety of materials ranging from hard (plastic, glass, metal, etc.) surfaces to cells on any substrate. It has been widely used to measure the stiffness of cells, but less frequently used to measure the stiffness of aortas. In this paper, we will describe the procedures for using AFM in contact mode to measure the ex vivo elastic modulus of unloaded mouse arteries. We describe our procedure for isolation of mouse aortas, and then provide detailed information for the AFM analysis. This includes step-by-step instructions for alignment of the laser beam, calibration of the spring constant and deflection sensitivity of the AFM probe, and acquisition of force curves. We also provide a detailed protocol for data analysis of the force curves.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.