ArticleScientific reports2016
Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
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- The effect of Legionella pneumophila infection on the encystation of Acanthamoeba castellanii.Parasites, hosts and diseases · 2026Article
- Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay.Nature communications · 2025Article
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- Amoebal Tubulin Cleavage Late during Infection Is a Characteristic Feature ofMicrobiology spectrum · 2022Article
- A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment.Nature communications · 2022Article
- MIP FromFrontiers in cellular and infection microbiology · 2022Article
- EdU Incorporation To Assess Cell Proliferation and Drug Susceptibility in Naegleria fowleri.Antimicrobial agents and chemotherapy · 2021Article
- The Ecology and Evolution of Amoeba-Bacterium Interactions.Applied and environmental microbiology · 2021Review
- Marine Bacteria Display Different Escape Mechanisms When Facing Their Protozoan Predators.Microorganisms · 2020Article
- Encystment Induces Down-Regulation of an Acetyltransferase-Like Gene inPathogens (Basel, Switzerland) · 2020Article
- Whole Organism Model to Study Molecular Mechanisms of Differentiation and Dedifferentiation.Biology · 2020Article
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- Mycoplasma hominis impacts gene expression in Trichomonas vaginalis.Parasitology research · 2018Article
- Differential expression of virulence genes in Legionella pneumophila growing in Acanthamoeba and human monocytes.Virulence · 2018Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella pneumophila is a ubiquitous, pathogenic, Gram-negative bacterium responsible for legionellosis. Like many other amoeba-resistant microorganisms, L. pneumophila resists host clearance and multiplies inside the cell. Through its Dot/Icm type IV secretion system, the bacterium injects more than three hundred effectors that modulate host cell physiology in order to promote its own intracellular replication. Here we report that L. pneumophila prevents proliferation of its natural host Acanthamoeba castellanii. Infected amoebae could not undergo DNA replication and no cell division was observed. The Dot/Icm secretion system was necessary for L. pneumophila to prevent the eukaryotic proliferation. The absence of proliferation was associated with altered amoebal morphology and with a decrease of mRNA transcript levels of CDC2b, a putative regulator of the A. castellanii cell cycle. Complementation of CDC28-deficient Saccharomyces cerevisiae by the CDC2b cDNA was sufficient to restore proliferation of CDC28-deficient S. cerevisiae and suggests for the first time that CDC2b from A. castellanii could be functional and a bona fide cyclin-dependent kinase. Hence, our results reveal that L. pneumophila impairs proliferation of A. castellanii and this effect could involve the cell cycle protein CDC2b.
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