Evidence map›Paper›PMID 27802194›Full record

ArticleCancer biomarkers : section A of Disease markers2016

Screening of exosomal microRNAs from colorectal cancer cells.

Cillian Clancy, Sonja Khan, Claire L Glynn, Emma Holian, Peter Dockery, Pierce Lalor, James A L Brown, Myles R Joyce, Michael J Kerin, Roisin M Dwyer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer biomarkers : section A of Disease markers, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Article
  2. Extracellular vesicles in colorectal cancer.Annals of coloproctology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Cillian ClancyDiscipline of Surgery, Lambe Institute for Translational Research, National University of Ireland Galway, Galway, Ireland.
Sonja KhanDiscipline of Surgery, Lambe Institute for Translational Research, National University of Ireland Galway, Galway, Ireland.
Claire L GlynnDiscipline of Surgery, Lambe Institute for Translational Research, National University of Ireland Galway, Galway, Ireland.
Emma HolianSchool of Mathematics, Statistics and Applied Mathematics, National University of Ireland Galway, Galway, Ireland.
Peter DockeryDiscipline of Anatomy, National University of Ireland Galway, Galway, Ireland.
Pierce LalorDiscipline of Anatomy, National University of Ireland Galway, Galway, Ireland.
James A L BrownDiscipline of Surgery, Lambe Institute for Translational Research, National University of Ireland Galway, Galway, Ireland.
Myles R JoyceDiscipline of Surgery, Lambe Institute for Translational Research, National University of Ireland Galway, Galway, Ireland.
Michael J KerinDiscipline of Surgery, Lambe Institute for Translational Research, National University of Ireland Galway, Galway, Ireland.
Roisin M DwyerDiscipline of Surgery, Lambe Institute for Translational Research, National University of Ireland Galway, Galway, Ireland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCells release extracellular membrane vesicles including microvesicles known as exosomes. Exosomes contain microRNAs (miRNAs) however the full range within colorectal cancer cell secreted exosomes is unknown.

objectiveTo identify the full range of exosome encapsulated miRNAs secreted from 2 colorectal cancer cell lines and to investigate engineering of exosomes over-expressing miRNAs.

methodsExosomes were isolated from HCT-116 and HT-29 cell lines. RNA was extracted from exosomes and microRNA array performed. Cells were engineered to express miR-379 (HCT-116-379) or a non-targeting control (HCT-116-NTC) and functional effects were determined. Exosomes secreted by engineered cells were transferred to recipient cells and the impact examined.

resultsMicrovesicles 40-100 nm in size secreted by cell lines were visualised and confirmed to express exosomal protein CD63. HT-29 exosomes contained 409 miRNAs, HCT-116 exosomes contained 393, and 338 were common to exosomes from both cell lines. Selected targets were validated. HCT-116-379 cells showed decreased proliferation (12-15% decrease, p < 0.001) and decreased migration (32-86% decrease, p < 0.001) compared to controls. HCT-116-379 exosomes were enriched for miR-379. Confocal microscopy visualised transfer of HCT-116-379 exosomes to recipient cells.

conclusionsColorectal cancer cells secrete a large number of miRNAs within exosomes. miR-379 decreases cell proliferation and migration, and miR-379 enriched exosomes can be engineered.

Indexed as

Cell Line, TumorCell ProliferationColorectal NeoplasmsExosomesHCT116 CellsHT29 CellsHumansMicroRNAsMicroRNAscolorectal cancerExosomesmicroRNAs

Identifiers

PMID27802194
PMCPMC13020523

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.