ArticleAnalytical chemistry2016
Label-Free Imaging of Histamine Mediated G Protein-Coupled Receptors Activation in Live Cells.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 19 citations in OpenAlex.
- In Situ and Label-Free Quantification of Membrane Protein-Ligand Interactions Using Optical Imaging Techniques: A Review.Biosensors · 2024Review
- Deep Learning Enhanced Label-Free Action Potential Detection Using Plasmonic-Based Electrochemical Impedance Microscopy.Analytical chemistry · 2024Article
- Plasmonic Scattering Microscopy for Label-Free Imaging of Molecular Binding Kinetics: From Single Molecules to Single Cells.Chemistry methods : new approaches to solving problems in chemistry · 2023Article
- Application of biosensors in cancers, an overview.Frontiers in bioengineering and biotechnology · 2023Review
- Plasmonic-based impedance microspectroscopy of optically heterogeneous samples.Biomedical optics express · 2020Article
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Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
G protein-coupled receptors (GPCRs) are the largest protein family for cell signal transduction, and most of them are crucial drug targets. Conventional label-free assays lack the spatial information to address the heterogeneous response from single cells after GPCRs activation. Here, we reported a GPCRs study in live cells using plasmonic-based electrochemical impedance microscopy. This label-free optical imaging platform is able to resolve responses from individual cells with subcellular resolution. Using this platform, we studied the histamine mediated GPCRs activation and revealed spatiotemporal heterogeneity of cellular downstream responses. Triphasic responses were observed from individual HeLa cells upon histamine stimulation. A quick peak P1 in less than 10 s was attributed to the GPCRs triggered calcium release. An inverted P2 phase within 1 min was attributed to the alternations of cell-matrix adhesion after the activation of Protein Kinase C (PKC). The main peak (P3) around 3-6 min after the histamine treatment was due to dynamic mass redistribution and showed a dose-dependent response with a half-maximal effective concentration (EC
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Registered trials
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