Evidence map›Paper›PMID 27800613›Full record

ArticleFEBS letters2016

Homodimerization enhances both sensitivity and dynamic range of the ligand-binding domain of type 1 metabotropic glutamate receptor.

Eugene Serebryany, Ewa Folta-Stogniew, Jian Liu, Elsa C Y Yan

Abstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Eugene SerebryanyDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.
Ewa Folta-StogniewW. M. Keck Foundation Biotechnology Resource Laboratory, Yale School of Medicine, Yale University, New Haven, CT, USA.
Jian LiuDepartment of Chemistry, Yale University, New Haven, CT, USA.
Elsa C Y YanDepartment of Chemistry, Yale University, New Haven, CT, USA.

Funding

Yale/NIDA Neuroproteomics Research CenterP30DA018343 · NIDA · YALE UNIVERSITY · PI Marina R Picciotto · 2004 to 2026
$37.1M
Asymmetric flow FFF and Composition Gradient/Light Scattering SystemS10RR023748 · NCRR · YALE UNIVERSITY · PI FOLTA-STOGNIEW, EWA JOANNA · 2007 to 2007
$271k
NCRR NIH HHS S10 RR023748NIDA NIH HHS P30 DA018343
6 · The paper itself

Abstract

Cooperativity in ligand binding is a key emergent property of protein oligomers. Positive cooperativity (higher affinity for subsequent binding events than for initial binding) is frequent. However, the symmetrically homodimeric ligand-binding domain (LBD) of metabotropic glutamate receptor type 1 exhibits negative cooperativity. To investigate its origin and functional significance, we measured the response to glutamate in vitro of wild-type and C140S LBD as a function of the extent of dimerization. Our results indicate that homodimerization enhances the affinity of the first, but not the second, binding site, relative to the monomer, giving the dimeric receptor both greater sensitivity and a broader dynamic range.

Indexed as

Protein MultimerizationAnimalsGlutamic AcidLigandsMiceModels, MolecularMutationProtein Structure, QuaternaryReceptors, Metabotropic GlutamateGlutamic AcidLigandsmetabotropic glutamate receptor type 1Receptors, Metabotropic GlutamatecooperativityG-protein coupled receptorligand bindinglight scatteringvenus flytrap fold

Identifiers

PMID27800613
PMCPMC5154874

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.