Evidence map›Paper›PMID 27800035›Full record

ArticleCellular and molecular bioengineering2016

Oncogene Knockdown via Active Loading of Small RNAs into Extracellular Vesicles by Sonication.

Tek N Lamichhane, Anjana Jeyaram, Divya B Patel, Babita Parajuli, Natalie K Livingston, Navein Arumugasaamy, John S Schardt, Steven M Jay

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular bioengineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 228 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
228citing papers in PubMed, 2 pooled it
7.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

228 citing papers in PubMed, 2 syntheses or guidelines pooled it, 367 citations in OpenAlex.

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168 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Tek N LamichhaneFischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
Anjana JeyaramFischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
Divya B PatelFischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
Babita ParajuliFischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
Natalie K LivingstonFischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
Navein ArumugasaamyFischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
John S SchardtFischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
Steven M JayFischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA; Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland, College Park, MD 20742 USA; Program in Molecular and Cell Biology, University of Maryland, College Park, MD 20742 USA.
University of Maryland, College Park · US

Funding

Engineering NRG Signaling For Therapeutic Vascularization In Diabetic WoundsR00HL112905 · NHLBI · UNIV OF MARYLAND, COLLEGE PARK · PI JAY, STEVEN MICHAEL · 2013 to 2015
$730k
Engineering NRG Signaling For Therapeutic Vascularization In Diabetic WoundsK99HL112905 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI JAY, STEVEN MICHAEL · 2012 to 2013
$182k
NHLBI NIH HHS K99 HL112905NHLBI NIH HHS R00 HL112905
6 · The paper itself

Abstract

Extracellular vesicles (EVs), including exosomes and microvesicles, have emerged as promising drug delivery vehicles for small RNAs (siRNA and miRNA) due to their natural role in intercellular RNA transport. However, the application of EVs for therapeutic RNA delivery may be limited by loading approaches that can induce cargo aggregation or degradation. Here, we report the use of sonication as a means to actively load functional small RNAs into EVs. Conditions under which EVs could be loaded with small RNAs with minimal detectable aggregation were identified, and EVs loaded with therapeutic siRNA via sonication were observed to be taken up by recipient cells and capable of target mRNA knockdown leading to reduced protein expression. This system was ultimately applied to reduce expression of HER2, an oncogenic receptor tyrosine kinase that critically mediates breast cancer development and progression, and could be extended to other therapeutic targets. These results define important parameters informing the application of sonication as a small RNA loading method for EVs and demonstrate the potential utility of this approach for versatile cancer therapy.

Indexed as

biopharmaceuticalbiotherapeuticcancer nanoparticleExosomesHER2microRNAnanobiotechnologysiRNA delivery

Identifiers

PMID27800035
PMCPMC5084850
OpenAlexW2472556431

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.