ArticleBiotechnology for biofuels2016
Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
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- Tandemly duplicated CYP82Ds catalyze 14-hydroxylation in triptolide biosynthesis and precursor production in Saccharomyces cerevisiae.Nature communications · 2023Article
- Genomic landscapes of bacterial transposons and their applications in strain improvement.Applied microbiology and biotechnology · 2022Review
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- Bornyl Diphosphate Synthase FromFrontiers in bioengineering and biotechnology · 2021Article
- Genome of Tripterygium wilfordii and identification of cytochrome P450 involved in triptolide biosynthesis.Nature communications · 2020Article
- Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches.Nature communications · 2018Article
- Biosynthesis and engineering of kaempferol in Saccharomyces cerevisiae.Microbial cell factories · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background
resultsAn efficient DNA assembly and chromosomal integration method was created by combining homologous recombination (HR) in
conclusionsModular design of transcription units and integration elements simplified assembly and integration procedure, and eliminated frequent designing and synthesis of DNA fragments in previous methods. Also, by assembling most parts in Step 1 in vitro, the number of DNA cassettes for homologous integration in Step 2 was significantly reduced. Thus, high assembly efficiency, high integration capacity, and low error rate were achieved.
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Registered trials
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