ArticleProceedings of the National Academy of Sciences of the United States of America2016
Mapping intracellular mechanics on micropatterned substrates.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Keratin intermediate filaments mechanically position melanin pigments for genome photoprotection.Nature cell biology · 2026Article
- Nucleo-cytoskeletal coupling controls intracellular deformation partitioning during cell stretching.Royal Society open science · 2025Article
- 3D Micropatterned Traction Force Microscopy: A Technique to Control 3D Cell Shape While Measuring Cell-Substrate Force Transmission.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Mechanical deformation and death of circulating tumor cells in the bloodstream.Cancer metastasis reviews · 2024Review
- Multi-Zone Visco-Node-Pore Sensing: A Microfluidic Platform for Multi-Frequency Viscoelastic Phenotyping of Single Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Article
- Mechanical properties of human tumour tissues and their implications for cancer development.Nature reviews. Physics · 2024Article
- Cytoplasmic viscosity is a potential biomarker for metastatic breast cancer cells.Nanoscale advances · 2024Article
- Trafficking in cancer: from gene deregulation to altered organelles and emerging biophysical properties.Frontiers in cell and developmental biology · 2024Review
- Biophysics in tumor growth and progression: from single mechano-sensitive molecules to mechanomedicine.Oncogene · 2023Review
- Friction patterns guide actin network contraction.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Engineering tools for quantifying and manipulating forces in epithelia.Biophysics reviews · 2023Review
- Extracellular Cues Govern Shape and Cytoskeletal Organization in Giant Unilamellar Lipid Vesicles.ACS synthetic biology · 2023Article
- Intrinsic cell rheology drives junction maturation.Nature communications · 2022Article
- Biophysical Approaches for Applying and Measuring Biological Forces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- A High Spatiotemporal Iontronic Single-Cell Viscometer.Research (Washington, D.C.) · 2022Article
- Passive and Active Microrheology for Biomedical Systems.Frontiers in bioengineering and biotechnology · 2022Review
- Passive coupling of membrane tension and cell volume during active response of cells to osmosis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Cell stiffness predicts cancer cell sensitivity to ultrasound as a selective superficial cancer therapy.Bioengineering & translational medicine · 2021Article
- Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
The mechanical properties of cells impact on their architecture, their migration, intracellular trafficking, and many other cellular functions and have been shown to be modified during cancer progression. We have developed an approach to map the intracellular mechanical properties of living cells by combining micropatterning and optical tweezers-based active microrheology. We optically trap micrometer-sized beads internalized in cells plated on crossbow-shaped adhesive micropatterns and track their displacement following a step displacement of the cell. The local intracellular complex shear modulus is measured from the relaxation of the bead position assuming that the intracellular microenvironment of the bead obeys power-law rheology. We also analyze the data with a standard viscoelastic model and compare with the power-law approach. We show that the shear modulus decreases from the cell center to the periphery and from the cell rear to the front along the polarity axis of the micropattern. We use a variety of inhibitors to quantify the spatial contribution of the cytoskeleton, intracellular membranes, and ATP-dependent active forces to intracellular mechanics and apply our technique to differentiate normal and cancer cells.
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