Evidence map›Paper›PMID 27798610›Full record

ArticleNature methods2016

Fluorescent indicators for simultaneous reporting of all four cell cycle phases.

Bryce T Bajar, Amy J Lam, Ryan K Badiee, Young-Hee Oh, Jun Chu, Xin X Zhou, Namdoo Kim, Benjamin B Kim, Mingyu Chung, Arielle L Yablonovitch and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers.

0numbers the graph read from it
0cells of the map it votes in
120citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

120 citing papers in PubMed.

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60 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Bryce T BajarDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Amy J LamDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Ryan K BadieeDepartment of Biological Sciences, Stanford University, Stanford, California, USA.
Young-Hee OhDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Jun ChuDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Xin X ZhouDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Namdoo KimDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Benjamin B KimDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Mingyu ChungDepartment of Chemical and Systems Biology, Stanford University, Stanford, California, USA.
Arielle L YablonovitchGraduate Program in Biophysics, Stanford University, Stanford, California, USA.
Barney F CruzDepartment of Chemistry, Stanford University, Stanford, California, USA.
Kanokwan KulalertDepartment of Chemical Engineering, Stanford University, Stanford, California, USA.
Jacqueline J TaoDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Tobias MeyerDepartment of Chemical and Systems Biology, Stanford University, Stanford, California, USA.
Xiao-Dong SuSchool of Life Sciences, Peking University, Beijing, China.
Michael Z LinDepartment of Bioengineering, Stanford University, Stanford, California, USA.

Funding

TRAINING & OUTREACH COREP50GM107615 · NIGMS · STANFORD UNIVERSITY · PI FERRELL, JAMES E. · 2013 to 2017
$10.9M
Molecular Biophysics Training Program at StanfordT32GM008294 · NIGMS · STANFORD UNIVERSITY · PI HUANG, KERWYN C. · 1989 to 2019
$7.4M
REGULATION OF CA++/CALMODULIN KINASE BY NEUROTRANSMITTERR01GM030179 · NIGMS · STANFORD UNIVERSITY · PI MEYER, TOBIAS · 1985 to 2018
$4.9M
Optogenetics for all: A general method for optical control of protein activityDP1GM111003 · NIGMS · STANFORD UNIVERSITY · PI LIN, MICHAEL Z. · 2013 to 2017
$4.0M
Decision points to enter and exit the human cell cycleR01GM118377 · NIGMS · STANFORD UNIVERSITY · PI MEYER, TOBIAS · 2016 to 2017
$566k
NIGMS NIH HHS DP1 GM111003NIGMS NIH HHS P50 GM107615NIGMS NIH HHS R01 GM030179NIGMS NIH HHS R01 GM118377NIGMS NIH HHS T32 GM008294
6 · The paper itself

Abstract

A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.

Indexed as

AnimalsCell Culture TechniquesCell CycleChromatinG2 PhaseHEK293 CellsHeLa CellsHumansLuminescent ProteinsMiceMitosisModels, MolecularMolecular ImagingNIH 3T3 CellsRecombinant Fusion ProteinsRed Fluorescent ProteinChromatinLuminescent ProteinsRecombinant Fusion ProteinsRed Fluorescent Protein

Identifiers

PMID27798610
PMCPMC5548384

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.