ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2016
Cell Line Macroarray: An Alternative High-Throughput Platform to Analyze hiPSC Lines.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Assessment of targets of antibody drug conjugates in SCLC.NPJ precision oncology · 2025Article
- The Application of the Tissue Microarray (TMA) Technology to Analyze Cerebral Organoids.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2021Article
- Slug Is A Surrogate Marker of Epithelial to Mesenchymal Transition (EMT) in Head and Neck Cancer.Journal of clinical medicine · 2020Article
- Siponimod (BAF312) Activates Nrf2 While Hampering NFκB in Human Astrocytes, and Protects From Astrocyte-Induced Neurodegeneration.Frontiers in immunology · 2020Article
- Immune co-culture cell microarray - a feasible tool for high-throughput functional investigation of lymphocyte-cancer interactions.Oncoimmunology · 2020Article
- Brain-Derived Neurotrophin and TrkB in Head and Neck Squamous Cell Carcinoma.International journal of molecular sciences · 2019Article
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10 authors.
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Abstract
In the past decade, tissue microarray (TMA) technology has evolved as an innovative tool for high-throughput proteomics analysis and mainly for biomarker validation. Similarly, enormous amount of data can be obtained from the cell line macroarray (CLMA) technology, which developed from the TMA using formalin-fixed, paraffin-embedded cell pellets. Here, we applied CLMA technology in stem cell research and in particular to identify bona fide neogenerated human induced pluripotent stem cell (hiPSC) clones suitable for down the line differentiation. All hiPSC protocols generate tens of clones, which need to be tested to determine genetically stable cell lines suitable for differentiation. Screening methods generally rely on fluorescence-activated cell sorting isolation and coverslip cell growth followed by immunofluorescence; these techniques could be cumbersome. Here, we show the application of CLMA to identify neogenerated pluripotent cell colonies and neuronal differentiated cell products. We also propose the use of the automated image analyzer, TissueQuest, as a reliable tool to quickly select the best clones, based upon the level of expression of multiple pluripotent biomarkers.
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