ArticleJournal of the American Chemical Society2016
Targeting Reactive Carbonyls for Identifying Natural Products and Their Biosynthetic Origins.
Article in Journal of the American Chemical Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
- Structural determination of natural products at speeds and scale of high-throughput screening.Natural product reports · 2026Review
- MALDI Matrix: Origins, Innovations, and Frontiers.Chemical reviews · 2026Review
- Unraveling the metabolic potential and roles of reductases in the omicsynin biosynthetic gene cluster.Natural products and bioprospecting · 2026Article
- Verazine biosynthesis from simple sugars in engineered Saccharomyces cerevisiae.Metabolic engineering · 2024Article
- The Complete Genomic Sequence of Microbial Transglutaminase Producer, Streptomyces mobaraensis DSM40587.Biochemical genetics · 2024Article
- Genomes and secondary metabolomes ofFrontiers in microbiology · 2024Article
- Genome mining unveils a class of ribosomal peptides with two amino termini.Nature communications · 2023Article
- Genome mining unveils a class of ribosomal peptides with two amino termini.bioRxiv : the preprint server for biology · 2023Article
- Streptomyces lividans 66 produces a protease inhibitor via a tRNA-utilizing enzyme interacting with a C-minus NRPS.Journal of industrial microbiology & biotechnology · 2023Article
- Identification of Isonitrile-Containing Natural Products in Complex Biological Matrices through Ligation with Chlorooximes.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
- Catch-enrich-release approach for amine-containing natural products.Chemical communications (Cambridge, England) · 2022Article
- Chimeric natural products derived from medermycin and the nature-inspired construction of their polycyclic skeletons.Nature communications · 2022Article
- Reference-Grade Genome and Large Linear Plasmid of Streptomyces rimosus: Pushing the Limits of Nanopore Sequencing.Microbiology spectrum · 2022Article
- ClpP inhibitors are produced by a widespread family of bacterial gene clusters.Nature microbiology · 2022Article
- Reactivity-based screening for natural product discovery.Methods in enzymology · 2022Article
- Enzymatic CJournal of the American Chemical Society · 2021Article
- Sterol Sponge Mechanism Is Conserved for Glycosylated Polyene Macrolides.ACS central science · 2021Article
- Article
- Reactivity-Based Screening for Citrulline-Containing Natural Products Reveals a Family of Bacterial Peptidyl Arginine Deiminases.ACS chemical biology · 2020Article
- Genome Mining and Metabolomics Uncover a Rare d-Capreomycidine Containing Natural Product and Its Biosynthetic Gene Cluster.ACS chemical biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Natural products (NPs) serve important roles as drug candidates and as tools for chemical biology. However, traditional NP discovery, largely based on bioassay-guided approaches, is biased toward abundant compounds and rediscovery rates are high. Orthogonal methods to facilitate discovery of new NPs are thus needed, and herein we describe an isotope tag-based expansion of reactivity-based NP screening to address these shortcomings. Reactivity-based screening is a directed discovery approach in which a specific reactive handle on the NP is targeted by a chemoselective probe to enable its detection by mass spectrometry. In this study, we have developed an aminooxy-containing probe to guide the discovery of aldehyde- and ketone-containing NPs. To facilitate the detection of labeling events, the probe was dibrominated, imparting a unique isotopic signature to distinguish labeled metabolites from spectral noise. As a proof of concept, the probe was then utilized to screen a collection of bacterial extracts, leading to the identification of a new analogue of antipain, deimino-antipain. The bacterial producer of deimino-antipain was sequenced and the responsible biosynthetic gene cluster was identified by bioinformatic analysis and heterologous expression. These data reveal the previously undetermined genetic basis for a well-known family of aldehyde-containing, peptidic protease inhibitors, including antipain, chymostatin, leupeptin, elastatinal, and microbial alkaline protease inhibitor, which have been widely used for over 40 years.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.