Evidence map›Paper›PMID 27797078›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis.

Jinbo Fan, Hui Jin, Hong-Guo Yu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jinbo FanDepartment of Biological Science, The Florida State University, Tallahassee, FL, 32306-4370, USA.
Hui JinDepartment of Biological Science, The Florida State University, Tallahassee, FL, 32306-4370, USA.
Hong-Guo YuDepartment of Biological Science, The Florida State University, Tallahassee, FL, 32306-4370, USA. hyu@bio.fsu.edu.
Florida State University · US

Funding

Molecular mechanism of centrosome separation in budding yeast meiosisR01GM117102 · NIGMS · FLORIDA STATE UNIVERSITY · PI YU, HONG-GUO · 2015 to 2018
$1.2M
NIGMS NIH HHS R01 GM117102
6 · The paper itself

Abstract

In this chapter, we describe a quantitative fluorescence-based assay of gene expression using the ratio of the reporter green fluorescence protein (GFP) to the internal red fluorescence protein (RFP) control. With this dual-color heterologous reporter assay, we have revealed cohesin-regulated genes and discovered a cis-acting DNA element, the Ty1-LTR, which interacts with cohesin and regulates gene expression during yeast meiosis. The method described here provides an effective cytological approach for quantitative analysis of global gene expression in budding yeast meiosis.

Indexed as

Cell Cycle ProteinsChromosomal Proteins, Non-HistoneChromosome SegregationCohesinsGene Expression Regulation, FungalGenes, ReporterGreen Fluorescent ProteinsLuminescent ProteinsMeiosisMolecular BiologyRed Fluorescent ProteinSaccharomyces cerevisiaeCell Cycle ProteinsChromosomal Proteins, Non-HistoneCohesinsGreen Fluorescent ProteinsLuminescent ProteinsRed Fluorescent ProteinCohesinFluorescence-based reporter assayGreen fluorescence proteinMeiosisRed fluorescence proteinTy1-LTR

Identifiers

PMID27797078
PMCPMC5551346
OpenAlexW2540806406

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.