Evidence map›Paper›PMID 27796930›Full record

ArticleJournal of microbiology (Seoul, Korea)2016

The assessment of host and bacterial proteins in sputum from active pulmonary tuberculosis.

Hsin-Chih Lai, Yu-Tze Horng, Pen-Fang Yeh, Jann-Yuan Wang, Chin-Chung Shu, Chia-Chen Lu, Jang-Jih Lu, Jen-Jyh Lee, Po-Chi Soo

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In one paragraph

Article in Journal of microbiology (Seoul, Korea), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Hsin-Chih LaiDepartment of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan, R.O.C.
Yu-Tze HorngDepartment of Laboratory Medicine and Biotechnology, Tzu Chi University, College of Medicine, Hualien, Taiwan, R.O.C.
Pen-Fang YehDepartment of Internal Medicine, Cheng Hsin Rehabilitation Medical Center, Taipei, Taiwan, R.O.C.
Jann-Yuan WangDepartment of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, R.O.C.
Chin-Chung ShuDepartment of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, R.O.C.
Chia-Chen LuDepartment of Respiratory Therapy, College of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan, R.O.C.
Jang-Jih LuGraduate Institute of Medical Biotechnology and Laboratory Science, College of Medicine, Chang-Gung University, Taoyuan, Taiwan, R.O.C.
Jen-Jyh LeeDepartment of Internal Medicine, Buddhist Tzu Chi General Hospital and Tzu Chi University, Hualien, Taiwan, R.O.C.
Po-Chi SooDepartment of Laboratory Medicine and Biotechnology, Tzu Chi University, College of Medicine, Hualien, Taiwan, R.O.C.. pcsoo@mail.tcu.edu.tw.
Tzu Chi University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pulmonary tuberculosis (TB) is caused by Mycobacterium tuberculosis. The protein composition of sputum may reflect the immune status of the lung. This study aimed to evaluate the protein profiles in spontaneous sputum samples from patients with active pulmonary TB. Sputum samples were collected from patients with pulmonary TB and healthy controls. Western blotting was used to analyze the amount of interleukin 10 (IL-10), interferon-gamma (IFN-γ), IL-25, IL-17, perforin-1, urease, albumin, transferrin, lactoferrin, adenosine deaminase (also known as adenosine aminohydrolase, or ADA), ADA-2, granzyme B, granulysin, and caspase-1 in sputum. Results of detection of IL-10, IFN-γ, perforin-1, urease, ADA2, and caspase-1, showed relatively high specificity in distinguishing patients with TB from healthy controls, although sensitivities varied from 13.3% to 66.1%. By defining a positive result as the detection of any two proteins in sputum samples, combined use of transferrin and urease as markers increased sensitivity to 73.2% and specificity to 71.1%. Furthermore, we observed that the concentration of transferrin was proportional to the number of acid-fast bacilli detected in sputum specimens. Detection of sputum transferrin and urease was highly associated with pulmonary TB infection. In addition, a high concentration of transferrin detected in sputum might correlate with active TB infection. This data on sputum proteins in patients with TB may aid in the development of biomarkers to assess the severity of pulmonary TB.

Indexed as

Host-Pathogen InteractionsAntigens, Differentiation, T-LymphocyteBacterial ProteinsBiomarkersBlotting, WesternFemaleHumansInterferon-gammaInterleukin-10Interleukin-17MaleMycobacterium tuberculosisProteinsSensitivity and SpecificitySputumTransferrinAntigens, Differentiation, T-LymphocyteBacterial ProteinsBiomarkersGNLY protein, humanIL10 protein, humanIL17A protein, humanInterferon-gammaInterleukin-10Interleukin-17ProteinsTransferrinUreaseacid-fast bacilliMycobacterium tuberculosissputumtransferrintuberculosisurease

Identifiers

PMID27796930
OpenAlexW2540100268

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.