ArticleAnalytical and bioanalytical chemistry2017
Quantitation of human milk proteins and their glycoforms using multiple reaction monitoring (MRM).
Article in Analytical and bioanalytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01817127 (Functional Deconstruction of Human Milk Oligosaccharides and Lipids), which is not on this map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Functional Deconstruction of Human Milk Oligosaccharides and Lipids
Who cites it
28 citing papers in PubMed.
- Lipid-Based Nutrient Supplements During Pregnancy and Lactation Did Not Affect Human Milk Oligosaccharides and Bioactive Proteins in a Randomized Trial.The Journal of nutrition · 2017Trial
- GP-Marker facilitates the analysis of intact glycopeptide quantitative data at different levels.Analytical and bioanalytical chemistry · 2025Article
- Multiple reaction monitoring assays for large-scale quantitation of proteins from 20 mouse organs and tissues.Communications biology · 2024Article
- Methods for quantification of glycopeptides by liquid separation and mass spectrometry.Mass spectrometry reviews · 2023Review
- Analysis of Minor Proteins Present in Breast Milk by Using WGA Lectin.Children (Basel, Switzerland) · 2022Article
- Article
- Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples.Nanomaterials (Basel, Switzerland) · 2021Article
- The Mothers, Infants, and Lactation Quality (MILQ) Study: A Multi-Center Collaboration.Current developments in nutrition · 2021Article
- Associations of Human Milk Oligosaccharides and Bioactive Proteins with Infant Morbidity and Inflammation in Malawian Mother-Infant Dyads.Current developments in nutrition · 2021Article
- Associations of human milk oligosaccharides and bioactive proteins with infant growth and development among Malawian mother-infant dyads.The American journal of clinical nutrition · 2021Article
- Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation.Molecular & cellular proteomics : MCP · 2021Review
- Survival of recombinant monoclonal and naturally-occurring human milk immunoglobulins A and G specific to respiratory syncytial virus F protein across simulated human infant gastrointestinal digestion.Journal of functional foods · 2020Article
- Therapeutic Monosaccharides: Looking Back, Moving Forward.Biochemistry · 2020Review
- Review
- NIH workshop on human milk composition: summary and visions.The American journal of clinical nutrition · 2019Article
- The Functional Power of the Human Milk Proteome.Nutrients · 2019Review
- Human Milk Proteins and Their Glycosylation Exhibit Quantitative Dynamic Variations during Lactation.The Journal of nutrition · 2019Article
- Validation and Application of Biocrates AbsoluteNutrients · 2019Article
- Article
- Peptidomic profiling of human milk with LC-MS/MS reveals pH-specific proteolysis of milk proteins.Food chemistry · 2019Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Human milk plays a substantial role in the child growth, development and determines their nutritional and health status. Despite the importance of the proteins and glycoproteins in human milk, very little quantitative information especially on their site-specific glycosylation is known. As more functions of milk proteins and other components continue to emerge, their fine-detailed quantitative information is becoming a key factor in milk research efforts. The present work utilizes a sensitive label-free MRM method to quantify seven milk proteins (α-lactalbumin, lactoferrin, secretory immunoglobulin A, immunoglobulin G, immunoglobulin M, α1-antitrypsin, and lysozyme) using their unique peptides while at the same time, quantifying their site-specific N-glycosylation relative to the protein abundance. The method is highly reproducible, has low limit of quantitation, and accounts for differences in glycosylation due to variations in protein amounts. The method described here expands our knowledge about human milk proteins and provides vital details that could be used in monitoring the health of the infant and even the mother. Graphical Abstract The glycopeptides EICs generated from QQQ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.