Evidence map›Paper›PMID 27796299›Full record

ArticleNature communications2016

Disposal of iron by a mutant form of lipocalin 2.

Jonathan Barasch, Maria Hollmen, Rong Deng, Eldad A Hod, Peter B Rupert, Rebecca J Abergel, Benjamin E Allred, Katherine Xu, Shaun F Darrah, Yared Tekabe and 12 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 43 citations in OpenAlex.

  1. Megalin: from structure to function.Nature reviews. Nephrology · 2026
    Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Lipocalin-2 and intestinal diseases.World journal of gastroenterology · 2024
    Review
  9. Spns1 is an iron transporter essential for megalin-dependent endocytosis.American journal of physiology. Renal physiology · 2024
    Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Review
  17. Review
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 5 institutions in 2 countries.

Jonathan BaraschColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Maria HollmenColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Rong DengColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Eldad A HodColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Peter B RupertFred Hutchinson Cancer Research Center, Basic Sciences Division, University of Washington School of Medicine Biochemistry, Immunology, Mail Stop A3-025, Seattle, Washington 98109, USA.
Rebecca J AbergelLawrence Berkeley National Laboratory, Chemical Sciences Division, BioActinide Chemistry Group, MS 70A-1150, One Cyclotron Road, Berkeley, California 94720, USA.
Benjamin E AllredLawrence Berkeley National Laboratory, Chemical Sciences Division, BioActinide Chemistry Group, MS 70A-1150, One Cyclotron Road, Berkeley, California 94720, USA.
Katherine XuColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Shaun F DarrahColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Yared TekabeColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Alan PerlsteinColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Rebecca WaxColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Efrat BruckColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Jacob StauberColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Kaitlyn A CorbinColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Charles BuchenColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Vesna SlavkovichColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Joseph GrazianoColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Steven L SpitalnikColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Guanhu BaoState Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China.
Roland K StrongFred Hutchinson Cancer Research Center, Basic Sciences Division, University of Washington School of Medicine Biochemistry, Immunology, Mail Stop A3-025, Seattle, Washington 98109, USA.
Andong QiuColumbia University, Russ Berrie Medical Science Pavilion, 1150 Saint Nicholas Avenue, Rm 411, New York, New York 10032, USA.
Columbia University · USFred Hutch Cancer Center · USLawrence Berkeley National Laboratory · USAnhui Agricultural University · CNTongji University · CN

Funding

True Metal fac coreP30ES009089 · NIEHS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI MATTHEW S PERZANOWSKI · 1998 to 2026
$44.7M
IRON LIPOCALIN NGAL IN KIDNEY DISEASER01DK073462 · NIDDK · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI BARASCH, JONATHAN M. · 2007 to 2015
$3.0M
Iron Delivery in the Developing KidneyR01DK092684 · NIDDK · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI BARASCH, JONATHAN M. · 2012 to 2016
$1.7M
Analysis of NGAL Metabolism Identifies a Therapy for Iron OverloadR21DK091729 · NIDDK · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI BARASCH, JONATHAN M. · 2011 to 2012
$437k
NIDDK NIH HHS R01 DK073462NIDDK NIH HHS R01 DK092684NIDDK NIH HHS R21 DK091729NIEHS NIH HHS P30 ES009089
6 · The paper itself

Abstract

Iron overload damages many organs. Unfortunately, therapeutic iron chelators also have undesired toxicity and may deliver iron to microbes. Here we show that a mutant form (K3Cys) of endogenous lipocalin 2 (LCN2) is filtered by the kidney but can bypass sites of megalin-dependent recapture, resulting in urinary excretion. Because K3Cys maintains recognition of its cognate ligand, the iron siderophore enterochelin, this protein can capture and transport iron even in the acidic conditions of urine. Mutant LCN2 strips iron from transferrin and citrate, and delivers it into the urine. In addition, it removes iron from iron overloaded mice, including models of acquired (iron-dextran or stored red blood cells) and primary (Hfe

Indexed as

AnimalsDisease Models, AnimalHumansInflammationIronIron Chelating AgentsIron OverloadKidneyLigandsLipocalin-2MiceMice, TransgenicMutationOxidation-ReductionProtein BindingSiderophoresIronIron Chelating AgentsLCN2 protein, humanLcn2 protein, mouseLigandsLipocalin-2SiderophoresTransferrin

Identifiers

PMID27796299
PMCPMC5095531
OpenAlexW2541295351

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.