Evidence map›Paper›PMID 27795849›Full record

ArticleActa naturae

Whole-Genome DNA Methylation Analysis of Peripheral Blood Mononuclear Cells in Multiple Sclerosis Patients with Different Disease Courses.

O G Kulakova, M R Kabilov, L V Danilova, E V Popova, O A Baturina, E Y Tsareva, N M Baulina, I S Kiselev, A N Boyko, A V Favorov and 2 more

Abstract read
PubMed Full text at PMC
In one paragraph

Article in Acta naturae. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Genetic and epigenetic signatures associated with plasma oxytocin levels in children and adolescents with autism spectrum disorder.Autism research : official journal of the International Society for Autism Research · 2023
    Trial
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. An Entropy Approach to Multiple Sclerosis Identification.Journal of personalized medicine · 2022
    Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Deciphering DNA Methylation in HIV Infection.Frontiers in immunology · 2021
    Review
  13. Review
  14. Review
  15. Environmental and genetic risk factors for MS: an integrated review.Annals of clinical and translational neurology · 2019
    Review
  16. Article
  17. Article
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

O G KulakovaPirogov Russian National Research Medical University, Ostrovityanova str. 1, Moscow, 117997, Russia ; Institute of Experimental Cardiology, Russian Cardiology Scientific and Production Center, 3th Cherepkovskaya str. 15A, Moscow, 121552, Russia.
M R KabilovInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Avenue 8, Novosibirsk, 630090, Russia.
L V DanilovaVavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina str. 1, Moscow, 119991 , Russia ; Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
E V PopovaPirogov Russian National Research Medical University, Ostrovityanova str. 1, Moscow, 117997, Russia.
O A BaturinaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Avenue 8, Novosibirsk, 630090, Russia.
E Y TsarevaPirogov Russian National Research Medical University, Ostrovityanova str. 1, Moscow, 117997, Russia ; Institute of Experimental Cardiology, Russian Cardiology Scientific and Production Center, 3th Cherepkovskaya str. 15A, Moscow, 121552, Russia.
N M BaulinaPirogov Russian National Research Medical University, Ostrovityanova str. 1, Moscow, 117997, Russia ; Institute of Experimental Cardiology, Russian Cardiology Scientific and Production Center, 3th Cherepkovskaya str. 15A, Moscow, 121552, Russia.
I S KiselevPirogov Russian National Research Medical University, Ostrovityanova str. 1, Moscow, 117997, Russia ; Institute of Experimental Cardiology, Russian Cardiology Scientific and Production Center, 3th Cherepkovskaya str. 15A, Moscow, 121552, Russia.
A N BoykoPirogov Russian National Research Medical University, Ostrovityanova str. 1, Moscow, 117997, Russia.
A V FavorovVavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina str. 1, Moscow, 119991 , Russia ; Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
O O FavorovaPirogov Russian National Research Medical University, Ostrovityanova str. 1, Moscow, 117997, Russia ; Institute of Experimental Cardiology, Russian Cardiology Scientific and Production Center, 3th Cherepkovskaya str. 15A, Moscow, 121552, Russia.
V V VlassovInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Avenue 8, Novosibirsk, 630090, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Multiple sclerosis (MS) is a severe neurodegenerative disease of polygenic etiology affecting the central nervous system. In addition to genetic factors, epigenetic mechanisms, primarily DNA methylation, which regulate gene expression, play an important role in MS development and progression. In this study, we have performed the first whole-genome DNA methylation profiling of peripheral blood mononuclear cells in relapsing-remitting MS (RRMS) and primary-progressive MS (PPMS) patients and compared them to those of healthy individuals in order to identify the differentially methylated CpG-sites (DMSs) associated with these common clinical disease courses. In addition, we have performed a pairwise comparison of DNA methylation profiles in RRMS and PPMS patients. All three pairwise comparisons showed significant differences in methylation profiles. Hierarchical clustering of the identified DMS methylation levels and principal component analysis for data visualization demonstrated a clearly defined aggregation of DNA samples of the compared groups into separate clusters. Compared with the control, more DMSs were identified in PPMS patients than in RRMS patients (67 and 30, respectively). More than half of DMSs are located in genes, exceeding the expected number for random distribution of DMSs between probes. RRMS patients mostly have hypomethylated DMSs, while in PPMS patients DMSs are mostly hypermethylated. CpG-islands and CpG-shores contain 60% of DMSs, identified by pairwise comparison of RRMS and control groups, and 79% of those identified by pairwise comparison of PPMS and control groups. Pairwise comparison of patients with two clinical MS courses revealed 51 DMSs, 82% of which are hypermethylated in PPMS. Overall, it was demonstrated that there are more changes in the DNA methylation profiles in PPMS than in RRMS. The data confirm the role of DNA methylation in MS development. We have shown, for the first time, that DNA methylation as an epigenetic mechanism is involved in the formation of two distinct clinical courses of MS: namely, RRMS and PPMS.

Indexed as

DNA methylationepigeneticsmultiple sclerosisperipheral blood mononuclear cells

Identifiers

PMID27795849
PMCPMC5081712

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.