ArticleJournal of biological engineering2016
Respiration activity monitoring system for any individual well of a 48-well microtiter plate.
Article in Journal of biological engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 64 citations in OpenAlex.
- Fluorescence-Based Online Monitoring Enables Differentiation in Co-Cultures of Untagged Streptomyces Species and Trichoderma reesei.Applied biochemistry and biotechnology · 2025Article
- Online monitoring of the mitochondrial respiration activity and protein formation in the Almost Living Cell-free Expression (ALiCE) system.BMC biotechnology · 2025Article
- Sigma Factors as Potential Targets to Enhance Recombinant Protein Expression.Biotechnology and bioengineering · 2025Article
- Scale-up of CHO cell cultures: from 96-well-microtiter plates to stirred tank reactors across three orders of magnitude.Journal of biological engineering · 2025Article
- Metabolic studies of Ogataea polymorpha using nine different corn steep liquors.BMC biotechnology · 2025Article
- Optimizing recombinant mini proinsulin production via response surface method and microbioreactor screening.PloS one · 2025Article
- Monitoring corn stover processing by the fungus Ustilago maydis.Bioresources and bioprocessing · 2024Article
- Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates.BMC biotechnology · 2023Article
- Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter plate.BMC biotechnology · 2023Article
- Assessing the capabilities of 2D fluorescence monitoring in microtiter plates with data-driven modeling for secondary substrate limitation experiments of Hansenula polymorpha.Journal of biological engineering · 2023Article
- Spotting priming-active compounds using parsley cell cultures in microtiter plates.BMC plant biology · 2023Article
- Insights intoEngineering in life sciences · 2023Article
- Development of a novel defined minimal medium for Gluconobacter oxydans 621H by systematic investigation of metabolic demands.Journal of biological engineering · 2022Article
- Tunable population dynamics in a synthetic filamentous coculture.MicrobiologyOpen · 2022Article
- Online 2D Fluorescence Monitoring in Microtiter Plates Allows Prediction of Cultivation Parameters and Considerable Reduction in Sampling Efforts for Parallel Cultivations ofBioengineering (Basel, Switzerland) · 2022Article
- Predicting high recombinant protein producer strains of Pichia pastoris MutScientific reports · 2022Article
- An illuminated respiratory activity monitoring system identifies priming-active compounds in plant seedlings.BMC plant biology · 2021Article
- Screening of a genome-reduced Corynebacterium glutamicum strain library for improved heterologous cutinase secretion.Microbial biotechnology · 2020Article
- Optimized polymer-based glucose release in microtiter plates for small-scaleJournal of biological engineering · 2020Article
- Polymer-based controlled-release fed-batch microtiter plate - diminishing the gap between early process development and production conditions.Journal of biological engineering · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSmall-scale micro-bioreactors have become the cultivation vessel of choice during the first steps of bioprocess development. They combine high cultivation throughput with enhanced cost efficiency per cultivation. To gain the most possible information in the early phases of process development, online monitoring of important process parameters is highly advantageous. One of these important process parameters is the oxygen transfer rate (OTR). Measurement of the OTR, however, is only available for small-scale fermentations in shake flasks via the established RAMOS technology until now. A microtiter plate-based (MTP) μRAMOS device would enable significantly increased cultivation throughput and reduced resource consumption. Still, the requirements of miniaturization for valve and sensor solutions have prevented this transfer so far. This study reports the successful transfer of the established RAMOS technology from shake flasks to 48-well microtiter plates. The introduced μRAMOS device was validated by means of one bacterial, one plant cell suspension culture and two yeast cultures.
resultsA technical solution for the required miniaturized valve and sensor implementation for an MTP-based μRAMOS device is presented. A microfluidic cover contains in total 96 pneumatic valves and 48 optical fibers, providing two valves and one optical fiber for each well. To reduce costs, an optical multiplexer for eight oxygen measuring instruments and 48 optical fibers is introduced. This configuration still provides a reasonable number of measurements per time and well. The well-to-well deviation is investigated by 48 identical
conclusionsThe introduced MTP-based μRAMOS device enables a sound and well resolved OTR monitoring for fast- and slow-growing organisms. It offers a quality similar to standard RAMOS in OTR determination combined with an easier handling. The experimental throughput is increased 6-fold and the media consumption per cultivation is decreased roughly 12.5-fold compared to the established eight shake flask RAMOS device.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.