ArticleMolecular and cellular biology1989
The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum utilizes alternate promoters and splicing for the synthesis of multiple mRNAs.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 41 citations in OpenAlex.
- Tomato polyphenol oxidase B is spatially and temporally regulated during development and in response to ethylene.Molecules (Basel, Switzerland) · 2011Article
- The Dictyostelium discoideum acaA gene is transcribed from alternative promoters during aggregation and multicellular development.PloS one · 2010Article
- Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP.The Biochemical journal · 2007Article
- Evolution of promoter sequences: elements of a canonical promoter for prespore genes of Dictyostelium.Journal of molecular evolution · 1996Article
- Dual role of cAMP during Dictyostelium development.Experientia · 1995Review
- Chemotactic sorting to cAMP in the multicellular stages of Dictyostelium development.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Identification of a protein kinase multigene family of Dictyostelium discoideum: molecular cloning and expression of a cDNA encoding a developmentally regulated protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- The molecular basis for alternative splicing of the CABP1 transcripts in Dictyostelium discoideum.Nucleic acids research · 1990Article
- Nuclear pre-mRNA introns: analysis and comparison of intron sequences from Tetrahymena thermophila and other eukaryotes.Nucleic acids research · 1990Article
- Two separable promoters control different aspects of expression of a Dictyostelium gene.Nucleic acids research · 1990Article
- The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum contains three promoters specific for growth, aggregation, and late development.Molecular and cellular biology · 1990Article
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
The cyclic nucleotide phosphodiesterase (phosphodiesterase) gene plays essential roles in the development of Dictyostelium discoideum during cellular aggregation and postaggregation morphogenesis. Genomic clones spanning the gene were isolated and used to determine the sequence and structure of the phosphodiesterase gene. We found an unusually complex organization for a gene of D. discoideum. Two transcripts of 2.4 and 1.9 kilobases (kb) were synthesized from start sites separated by 1.1 kb. A developmentally regulated promoter was utilized for the 2.4-kb mRNA, and a constitutive promoter regulated synthesis of the 1.9-kb transcript. The gene was found to be divided into four exons that are alternately spliced to give rise to the two mRNAs. The precursor of the 2.4-kb mRNA contained a 2.3-kb intron, whereas the precursor of the constitutive transcript was synthesized with a 1.7-kb intron. The two transcripts contained identical protein-coding regions and 400-nucleotide 3' untranslated sequences. The 2.4-kb developmentally regulated mRNA was distinguished by a long 5' untranslated leader of 666 nucleotides. The complex structure of the gene may allow multiple levels of control of the expression of the phosphodiesterase during development.
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