ArticleInfection and immunity2017
Purine Biosynthesis Metabolically Constrains Intracellular Survival of Uropathogenic Escherichia coli.
Article in Infection and immunity, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 79 citations in OpenAlex.
- Article
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- De novo or Salvage? Nucleotide Availability as a Driver of Bacterial Adaptation and Virulence.MicrobiologyOpen · 2026Review
- A requirement formSphere · 2026Article
- A short peptide derived from late embryogenesis abundant proteins enhances acid tolerance in Escherichia coli via modulation of two-component regulatory systems.The FEBS journal · 2026Article
- Genomic and functional characterization of the L‑sorbose phosphotransferase system in high-riskmSystems · 2026Article
- De novo nucleotide biosynthesis and its dynamic regulation are crucial for systemic infection by extraintestinal Escherichia coli.PLoS pathogens · 2026Article
- Transcriptional changes related to metabolic and iron acquisition strategies of avian pathogenicFrontiers in cellular and infection microbiology · 2026Article
- IL-1β alters the virulence of uropathogenic Escherichia coli.Scientific reports · 2025Article
- Role of GuaB, the inosine-5'-monophosphate dehydrogenase of uropathogenicMicrobiology spectrum · 2025Article
- The impact of bacterial purine metabolism on antibiotic efficacy.npj antimicrobials and resistance · 2025Review
- Beyond anaerobic respiration-new physiological roles for DmsABC and other S-/N-oxide reductases inJournal of bacteriology · 2025Article
- A thiouracil desulfurase protects Clostridioides difficile RNA from 4-thiouracil incorporation, providing a competitive advantage in the gut.Cell host & microbe · 2025Article
- Low leucine levels in the blood enhance the pathogenicity of neonatal meningitis-causing Escherichia coli.Nature communications · 2025Article
- Differential effects of inosine monophosphate dehydrogenase (IMPDH/GuaB) inhibition inJournal of bacteriology · 2024Article
- Genetic requirements for uropathogenicmSystems · 2024Article
- Lactate promotes the biofilm-to-invasive-planktonic transition inInfection and immunity · 2024Article
- Metabolomics analysis of the lactobacillus plantarum ATCC 14917 response to antibiotic stress.BMC microbiology · 2024Article
- Environmental purines decrease Pseudomonas aeruginosa biofilm formation by disrupting c-di-GMP metabolism.Cell reports · 2024Article
- Raising the alarm: fosfomycin resistance associated with non-susceptible inner colonies imparts no fitness cost to the primary bacterial uropathogen.Antimicrobial agents and chemotherapy · 2024Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
The ability to de novo synthesize purines has been associated with the intracellular survival of multiple bacterial pathogens. Uropathogenic Escherichia coli (UPEC), the predominant cause of urinary tract infections, undergoes a transient intracellular lifestyle during which bacteria clonally expand into multicellular bacterial communities within the cytoplasm of bladder epithelial cells. Here, we characterized the contribution of the conserved de novo purine biosynthesis-associated locus cvpA-purF to UPEC pathogenesis. Deletion of cvpA-purF, or of purF alone, abolished de novo purine biosynthesis but did not impact bacterial adherence properties in vitro or in the bladder lumen. However, upon internalization by bladder epithelial cells, UPEC deficient in de novo purine biosynthesis was unable to expand into intracytoplasmic bacterial communities over time, unless it was extrachromosomally complemented. These findings indicate that UPEC is deprived of purine nucleotides within the intracellular niche and relies on de novo purine synthesis to meet this metabolic requirement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.