Evidence map›Paper›PMID 27795210›Full record

ArticleGenome biology2016

Rapid gene isolation in barley and wheat by mutant chromosome sequencing.

Javier Sánchez-Martín, Burkhard Steuernagel, Sreya Ghosh, Gerhard Herren, Severine Hurni, Nikolai Adamski, Jan Vrána, Marie Kubaláková, Simon G Krattinger, Thomas Wicker and 3 more

Abstract read
In one paragraph

Article in Genome biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 176 papers.

0numbers the graph read from it
0cells of the map it votes in
176citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

176 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Fine mapping and candidate gene dissection of the wheat powdery mildew resistance gene PmPBDH.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Genetic Basis Identification of aPlants (Basel, Switzerland) · 2025
    Article
  17. Article
  18. Article
  19. Fine mapping of a novel powdery mildew resistance gene PmDM8 derived from a cultivated emmer (Triticum dicoccum).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Article
  20. Article

116 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Javier Sánchez-MartínDepartment of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland.
Burkhard SteuernagelJohn Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
Sreya GhoshJohn Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
Gerhard HerrenDepartment of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland.
Severine HurniDepartment of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland.
Nikolai AdamskiJohn Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
Jan VránaInstitute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, Olomouc, CZ-78371, Czech Republic.
Marie KubalákováInstitute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, Olomouc, CZ-78371, Czech Republic.
Simon G KrattingerDepartment of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland.
Thomas WickerDepartment of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland.
Jaroslav DoleželInstitute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, Olomouc, CZ-78371, Czech Republic.
Beat KellerDepartment of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland. bkeller@botinst.uzh.ch.
Brande B H WulffJohn Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK. brande.wulff@jic.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.

Indexed as

Chromosomes, PlantCloning, MolecularGenes, PlantMutationHordeumPhenotypePolymorphism, Single NucleotideSequence Analysis, DNATriticumBarleyChromosome flow sortingGene cloningMutational genomicsMutChromSeqTriticeaeWheat

Identifiers

PMID27795210
PMCPMC5087116

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.