ArticleBMC biotechnology2016
Improving the active expression of transglutaminase in Streptomyces lividans by promoter engineering and codon optimization.
Article in BMC biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 42 citations in OpenAlex.
- Rewiring Amino Acid Flux for Efficient Transglutaminase Production inJournal of agricultural and food chemistry · 2026Article
- Engineering genetic elements for microbial protein expression systems: Advances, challenges, applications, and prospects.Synthetic and systems biotechnology · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Microbial transglutaminase in food biotechnology: from biochemical mechanisms to industrial applications.Applied microbiology and biotechnology · 2026Review
- Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes.Applied microbiology and biotechnology · 2024Review
- LexA, an SOS response repressor, activates TGase synthesis inFrontiers in microbiology · 2024Article
- Microbiological transglutaminase: Biotechnological application in the food industry.Open life sciences · 2023Review
- CRISPR/Cas9-mediated genome editing in vancomycin-producing strainFrontiers in bioengineering and biotechnology · 2023Article
- Novel Alkaloids from Marine Actinobacteria: Discovery and Characterization.Marine drugs · 2021Review
- Review
- Targeted Genome Mining-From Compound Discovery to Biosynthetic Pathway Elucidation.Microorganisms · 2020Article
- Heterologous Expression of Recombinant Transglutaminase inJournal of microbiology and biotechnology · 2020Article
- Coordinated regulation for nature products discovery and overproduction inSynthetic and systems biotechnology · 2020Article
- A recent update on the use of microbial transglutaminase for the generation of biotherapeutics.World journal of microbiology & biotechnology · 2020Review
- Transglutaminases: part I-origins, sources, and biotechnological characteristics.World journal of microbiology & biotechnology · 2020Review
- Streptomycetes: Attractive Hosts for Recombinant Protein Production.Frontiers in microbiology · 2020Review
- A novel autolysis system for extracellular production and direct immobilization of a phospholipase D fused with cellulose binding domain.BMC biotechnology · 2019Article
- The Transcription Unit Architecture ofFrontiers in microbiology · 2019Article
- Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein.Microbial cell factories · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTransglutaminases (TGase), which are synthesized as a zymogen (pro-TGase) in Streptomyces sp., are important enzymes in the food industry. Because this pro-peptide is essential for the correct folding of Streptomyces TGase, TGase is usually expressed in an inactive pro-TGase form, which is then converted to active TGase by the addition of activating proteases in vitro. In this study, Streptomyces hygroscopicus TGase was actively produced by Streptomyces lividans through promoter engineering and codon optimization.
resultsA gene fragment (tg1, 2.6 kb) that encoded the pro-TGase and its endogenous promoter region, signal peptide and terminator was amplified from S. hygroscopicus WSH03-13 and cloned into plasmid pIJ86, which resulted in pIJ86/tg1. After fermentation for 2 days, S. lividans TK24 that harbored pIJ86/tg1 produced 1.8 U/mL of TGase, and a clear TGase band (38 kDa) was detected in the culture supernatant. These results indicated that the pro-TGase was successfully expressed and correctly processed into active TGase in S. lividans TK24 by using the TGase promoter. Based on deletion analysis, the complete sequence of the TGase promoter is restricted to the region from -693 to -48. We also identified a negative element (-198 to -148) in the TGase promoter, and the deletion of this element increased the TGase production by 81.3 %, in contrast to the method by which S. lividans expresses pIJ86/tg1. Combining the deletion of the negative element of the promoter and optimization of the gene codons, the yield and productivity of TGase reached 5.73 U/mL and 0.14 U/mL/h in the recombinant S. lividans, respectively.
conclusionsWe constructed an active TGase-producing strain that had a high yield and productivity, and the optimized TGase promoter could be a good candidate promoter for the expression of other proteins in Streptomyces.
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