ArticleMicrobial cell factories2016
Evaluation of gene expression cassettes and production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with a fine modulated monomer composition by using it in Cupriavidus necator.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 53 citations in OpenAlex.
- An Oxygen-Tolerant Archaeal Dehydratase Enables a Synthetic Pathway for 4-Hydroxybutyrate-Containing Polyesters from Glucose and COACS synthetic biology · 2026Article
- Controlling 3-hydroxyhexanoate mole fraction in poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) by altering enoyl-CoA hydratase (phaJ) ribosome-binding site in Cupriavidus necator H16.Bioprocess and biosystems engineering · 2026Article
- Metabolic Engineering Strategies for Enhanced Polyhydroxyalkanoate (PHA) Production inPolymers · 2025Review
- Efficient Production of High-Concentration Poly(3-hydroxybutyrate-Bioengineering (Basel, Switzerland) · 2025Article
- Unlocking the potential of Cupriavidus necator H16 as a platform for bioproducts production from carbon dioxide.World journal of microbiology & biotechnology · 2024Review
- Novel differential scanning calorimetry (DSC) application to select polyhydroxyalkanoate (PHA) producers correlating 3-hydroxyhexanoate (3-HHx) monomer with melting enthalpy.Bioprocess and biosystems engineering · 2024Article
- Synthetic biology toolkit of Ralstonia eutropha (Cupriavidus necator).Applied microbiology and biotechnology · 2024Review
- Identification of Oil-LovingPolymers · 2024Article
- High-cell-density fed-batch strategy to manufacture tailor-made P(HB-co-HHx) by engineered Ralstonia eutropha at laboratory scale and pilot scale.Microbial biotechnology · 2024Article
- Expanding the synthetic biology toolbox of Cupriavidus necator for establishing fatty acid production.Journal of industrial microbiology & biotechnology · 2024Article
- Key challenges in the advancement and industrialization of biobased and biodegradable plastics: a value chain overarching perspective.Frontiers in bioengineering and biotechnology · 2024Article
- Continuous feeding strategy for polyhydroxyalkanoate production from solid waste animal fat at laboratory- and pilot-scale.Microbial biotechnology · 2023Article
- Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant BacteriumACS synthetic biology · 2022Article
- Impact of various β-ketothiolase genes on PHBHHx production in Cupriavidus necator H16 derivatives.Applied microbiology and biotechnology · 2022Article
- Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for COBiotechnology for biofuels · 2021Review
- Effects of the Monomeric Components of Poly-hydroxybutyrate-co-hydroxyhexanoate on the Growth ofAnimals : an open access journal from MDPI · 2021Article
- Engineering ofFrontiers in bioengineering and biotechnology · 2021Article
- Substrate-Flexible Two-Stage Fed-Batch Cultivations for the Production of the PHA Copolymer P(HB-Frontiers in bioengineering and biotechnology · 2021Article
- A marine photosynthetic microbial cell factory as a platform for spider silk production.Communications biology · 2020Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCupriavidus necator has attracted much attention as a platform for the production of polyhydroxyalkanoate (PHA) and other useful materials. Therefore, an appropriate modulation of gene expression is needed for producing the desired materials effectively. However, there is insufficient information on the genetic engineering techniques required for this in C. necator.
resultsWe found that the disruption of a potential ribosome binding site (RBS) in the phaC1 gene in C. necator caused a small decrease in the PhaC1 expression level. We applied this result to finely regulate the expression of other genes. Several gene expression cassettes were constructed by combining three Escherichia coli derived promoters (P
conclusionsWe constructed and evaluated several gene expression cassettes consisting of promoters and RBSs that finely regulate transcription and translation. These were then applied to finely modulate the monomer composition in the production of PHBHHx by recombinant C. necator.
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