ArticleInternational journal of molecular sciences2016
iTRAQ-Based Quantitative Proteomic Analysis of the Potentiated and Dormant Antler Stem Cells.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Characterizing therapeutic effects of velvet antler using different omics strategies.Journal of traditional and complementary medicine · 2025Review
- Mining key circRNA-associated-ceRNA networks for milk fat metabolism in cows with varying milk fat percentages.BMC genomics · 2024Article
- Haplotype-resolved Genome of Sika Deer Reveals Allele-specific Gene Expression and Chromosome Evolution.Genomics, proteomics & bioinformatics · 2023Article
- MiRNA Profiling and Its Potential Roles in Rapid Growth of Velvet Antler in Gansu Red Deer (Genes · 2023Article
- Integrative Analyses of Antler Cartilage Transcriptome and Proteome of Gansu Red Deer (Animals : an open access journal from MDPI · 2022Article
- Calreticulin Identified as One of the Androgen Response Genes That Trigger Full Regeneration of the Only Capable Mammalian Organ, the Deer Antler.Frontiers in cell and developmental biology · 2022Article
- The periosteum: a simple tissue with many faces, with special reference to the antler-lineage periostea.Biology direct · 2021Review
- SWATH-MS Quantitative Proteomic Analysis of Deer Antler from Two Regenerating and Mineralizing Sections.Biology · 2021Article
- Article
- iTRAQ-Based Quantitative Proteomic Profiling ofFrontiers in microbiology · 2019Article
- Proteomic Analysis of Plasma Membrane Proteins of Antler Stem Cells Using Label-Free LC⁻MS/MS.International journal of molecular sciences · 2018Article
- A proteomic approach reveals the differential protein expression inJournal of ginseng research · 2018Article
- iTRAQ-Based Identification of Proteins Related to Muscle Growth in the Pacific Abalone, Haliotis discus hannai.International journal of molecular sciences · 2017Article
Corrections and comments
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
As the only known organ that can completely regenerate in mammals, deer antler is of real significance in the field of regenerative medicine. Recent studies have shown that the regenerative capacity of the antlers comes from the pedicle periosteum and the cells resident in the periosteum possess the attributes of stem cells. Currently, the molecular mechanism of antler regeneration remains unclear. In the present study, we compared the potentiated and dormant antler stem cells using isobaric tags for the relative and absolute quantification (iTRAQ) labeling of the peptides, coupled with two-dimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS) to compare the proteome profiles. Proteins were identified by searching against the NCBI nr database and our own Cervine transcriptome database, and bioinformatics analysis was conducted to identify the differentially expressed proteins. Based on this searching strategy, we identified 169 differentially expressed proteins in total, consisting of 70 up- and 99 down-regulated in the potentiated vs. dormant antler stem cells. Reliability of the iTRAQ was confirmed via quantitative real-time polymerase chain reaction (qRT-PCR) to measure the expression of selected genes. We identified transduction pathways through the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, such as HIF-1 and PI3K-AKT signaling pathways that play important roles in regulating the regeneration of antlers. In summary, the initiation stage of antler regeneration, a process from dormant to potentiated states in antler stem cells, is regulated by multiple proteins and complicated signal networks.
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