ReviewBioengineered2017
The growing impact of lyophilized cell-free protein expression systems.
Review in Bioengineered, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Microbial cell-free protein synthesis and its progression toward industrial use.Microbiology (Reading, England) · 2026Review
- International multisite implementation of distributed cell-free protein biomanufacturing to advance health and research equity.Science advances · 2026Article
- Recent Advances in Developing Cell-Free Protein Synthesis Biosensors for Medical Diagnostics and Environmental Monitoring.Biosensors · 2025Review
- Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Cell Free Bacteriophage Synthesis from Engineered Strains Improves Yield.ACS synthetic biology · 2023Article
- A Low-Cost, Thermostable, Cell-Free Protein Synthesis Platform for On-Demand Production of Conjugate Vaccines.ACS synthetic biology · 2023Article
- Solid-Phase Cell-Free Protein Synthesis and Its Applications in Biotechnology.Advances in biochemical engineering/biotechnology · 2023Article
- A low-cost recombinant glycoconjugate vaccine confers immunogenicity and protection against enterotoxigenicFrontiers in molecular biosciences · 2023Article
- Predicting Protein-Ligand Docking Structure with Graph Neural Network.Journal of chemical information and modeling · 2022Review
- Effects of DNA template preparation on variability in cell-free protein production.Synthetic biology (Oxford, England) · 2022Article
- Best Practices for DNA Template Preparation Toward Improved Reproducibility in Cell-Free Protein Production.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction.Frontiers in bioengineering and biotechnology · 2022Article
- Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles.Nature communications · 2021Article
- Development of a robustBiochemical engineering journal · 2021Article
- Methodologies for preparation of prokaryotic extracts for cell-free expression systems.Synthetic and systems biotechnology · 2020Review
- A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli.Cell chemical biology · 2019Article
- Streamlining the preparation of "endotoxin-free" ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase.Synthetic and systems biotechnology · 2019Article
- Cell-Free Metabolic Engineering: Recent Developments and Future Prospects.Methods and protocols · 2019Review
- Optimizing Cell-Free Protein Synthesis for Increased Yield and Activity of Colicins.Methods and protocols · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently reported shelf-stable, on-demand protein synthesis platforms are enabling new possibilities in biotherapeutics, biosensing, biocatalysis, and high throughput protein expression. Lyophilized cell-free protein expression systems not only overcome cold-storage limitations, but also enable stockpiling for on-demand synthesis and completely sterilize the protein synthesis platform. Recently reported high-yield synthesis of cytotoxic protein Onconase from lyophilized E. coli extract preparations demonstrates the utility of lyophilized cell-free protein expression and its potential for creating on-demand biotherapeutics, vaccines, biosensors, biocatalysts, and high throughput protein synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.