ArticleAnalytical chemistry2016
Secondary Structure and Glycosylation of Mucus Glycoproteins by Raman Spectroscopies.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- A Coarse-Grained MARTINI Model for Mucins.Journal of chemical theory and computation · 2026Article
- HAMA-SBMA hydrogel with anti-inflammatory properties delivers cartilage organoids, boosting cartilage regeneration.Journal of nanobiotechnology · 2025Article
- Neuraminidase-mediated enhancement ofmBio · 2025Article
- Glycosylation: mechanisms, biological functions and clinical implications.Signal transduction and targeted therapy · 2024Review
- Investigation of Hydrocolloid Plant Polysaccharides as Potential Candidates to Mimic the Functions of MUC5B in Saliva.Pharmaceutics · 2024Article
- Self-Assembling Polypeptides in Complex Coacervation.Accounts of chemical research · 2024Article
- Direct observation of glycans bonded to proteins and lipids at the single-molecule level.Science (New York, N.Y.) · 2023Article
- Mirror Image Mucins and Thio Mucins with Tunable Biodegradation.Journal of the American Chemical Society · 2023Article
- The lipophilic cyclic peptide cyclosporin A induces aggregation of gel-forming mucins.Scientific reports · 2022Article
- Montmorillonite stabilized chitosan-RSC advances · 2021Article
- Lubricating properties of chewing stimulated whole saliva from patients suffering from xerostomia.Clinical oral investigations · 2021Article
- Paving the way to conformationally unravel complex glycopeptide antibiotics by means of Raman optical activity.Chemical science · 2021Article
- The Biological Synthesis and the Function of Mucin 2 in Pseudomyxoma Peritonei.Cancer management and research · 2021Review
- Intestinal inflammation alters mucosal carbohydrate foraging and monosaccharide incorporation into microbial glycans.Cellular microbiology · 2021Article
- The MUC5B mucin polymer is dominated by repeating structural motifs and its topology is regulated by calcium and pH.Scientific reports · 2019Article
- The C-terminal dimerization domain of the respiratory mucin MUC5B functions in mucin stability and intracellular packaging before secretion.The Journal of biological chemistry · 2019Article
- Shedding Light on the Trehalose-Enabled Mucopermeation of Nanoparticles with Label-Free Raman Spectroscopy.Small (Weinheim an der Bergstrasse, Germany) · 2019Article
- Nanoscale chemical imaging using tip-enhanced Raman spectroscopy.Nature protocols · 2019Article
- Review
- Probing Membrane Receptors with Enhanced Raman Imaging.Proceedings of SPIE--the International Society for Optical Engineering · 2018Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 5 countries.
Funding
Abstract
The major structural components of protective mucus hydrogels on mucosal surfaces are the secreted polymeric gel-forming mucins. The very high molecular weight and extensive O-glycosylation of gel-forming mucins, which are key to their viscoelastic properties, create problems when studying mucins using conventional biochemical/structural techniques. Thus, key structural information, such as the secondary structure of the various mucin subdomains, and glycosylation patterns along individual molecules, remains to be elucidated. Here, we utilized Raman spectroscopy, Raman optical activity (ROA), circular dichroism (CD), and tip-enhanced Raman spectroscopy (TERS) to study the structure of the secreted polymeric gel-forming mucin MUC5B. ROA indicated that the protein backbone of MUC5B is dominated by unordered conformation, which was found to originate from the heavily glycosylated central mucin domain by isolation of MUC5B O-glycan-rich regions. In sharp contrast, recombinant proteins of the N-terminal region of MUC5B (D1-D2-D'-D3 domains, NT5B), C-terminal region of MUC5B (D4-B-C-CK domains, CT5B) and the Cys-domain (within the central mucin domain of MUC5B) were found to be dominated by the β-sheet. Using these findings, we employed TERS, which combines the chemical specificity of Raman spectroscopy with the spatial resolution of atomic force microscopy to study the secondary structure along 90 nm of an individual MUC5B molecule. Interestingly, the molecule was found to contain a large amount of α-helix/unordered structures and many signatures of glycosylation, pointing to a highly O-glycosylated region on the mucin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.