ArticleSoft matter2016
Biophysical characterization of organelle-based RNA/protein liquid phases using microfluidics.
Article in Soft matter, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Liquid-Liquid Phase Separation (LLPS) in Tumor Biology: Special Focus on the Process of Transcription.International journal of molecular sciences · 2026Review
- The rheology and interfacial properties of biomolecular condensates.Biophysical reviews · 2025Review
- Live-cell quantification reveals viscoelastic regulation of synapsin condensates by α-synuclein.Science advances · 2025Article
- Microphase separation produces interfacial environment within diblock biomolecular condensates.eLife · 2025Article
- Direct computations of viscoelastic moduli of biomolecular condensates.The Journal of chemical physics · 2024Article
- Fundamental Aspects of Phase-Separated Biomolecular Condensates.Chemical reviews · 2024Review
- ATP-induced cross-linking of a biomolecular condensate.Biophysical journal · 2024Article
- A calibration-free model of micropipette aspiration for measuring properties of protein condensates.Biophysical journal · 2024Article
- Biomolecular condensates form spatially inhomogeneous network fluids.Nature communications · 2024Article
- Article
- Liquid spherical shells are a non-equilibrium steady state of active droplets.Nature communications · 2023Article
- The Rous sarcoma virus Gag Polyprotein Forms Biomolecular Condensates Driven by Intrinsically-disordered Regions.Journal of molecular biology · 2023Article
- Improving the hole picture: towards a consensus on the mechanism of nuclear transport.Biochemical Society transactions · 2023Article
- ATP-induced crosslinking of a biomolecular condensate.bioRxiv : the preprint server for biology · 2023Article
- Dynamic molecular mechanism of the nuclear pore complex permeability barrier.bioRxiv : the preprint server for biology · 2023Article
- Article
- Biomolecular condensate phase diagrams with a combinatorial microdroplet platform.Nature communications · 2022Article
- Microfluidics for multiscale studies of biomolecular condensates.Lab on a chip · 2022Review
- Evolution and Single-Droplet Analysis of Fuel-Driven Compartments by Droplet-Based Microfluidics.Angewandte Chemie (International ed. in English) · 2022Article
- Dynamic arrest and aging of biomolecular condensates are modulated by low-complexity domains, RNA and biochemical activity.Nature communications · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Living cells contain numerous membrane-less RNA/protein (RNP) bodies that assemble by intracellular liquid-liquid phase separation. The properties of these condensed phase droplets are increasingly recognized as important in their physiological function within living cells, and also through the link to protein aggregation pathologies. However, techniques such as droplet coalescence analysis or standard microrheology do not always enable robust property measurements of model RNA/protein droplets in vitro. Here, we introduce a microfluidic platform that drives protein droplets into a single large phase, which facilitates viscosity measurements using passive microrheology and/or active two-phase flow analysis. We use this technique to study various phase separating proteins from structures including P granules, nucleoli, and Whi3 droplets. In each case, droplets exhibit simple liquid behavior, with shear rate-independent viscosities, over observed timescales. Interestingly, we find that a reported order of magnitude difference between the timescale of Whi3 and LAF-1 droplet coalescence is driven by large differences in surface tension rather than viscosity, with implications for droplet assembly and function. The ability to simultaneously perform active and passive microrheological measurements enables studying the impact of ATP-dependent biological activity on RNP droplets, which is a key area for future research.
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