Evidence map›Paper›PMID 27791182›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2016

Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation.

Xin Jiang, Irina Smirnova, Vladimir Kasho, Jianping Wu, Kunio Hirata, Meng Ke, Els Pardon, Jan Steyaert, Nieng Yan, H Ronald Kaback

Open access · bronzeAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
3.7field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 41 citations in OpenAlex.

  1. Conformational free-energy landscapes of a NaProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  2. Article
  3. Production of Single-Domain Antibodies in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  4. Article
  5. Function Trumps Form in Two Sugar Symporters,International journal of molecular sciences · 2021
    Review
  6. Review
  7. Plant glucose transporter structure and function.Pflugers Archiv : European journal of physiology · 2020
    Review
  8. H. Ronald Kaback 1936-2019.Nature structural & molecular biology · 2020
    Article
  9. The proton electrochemical gradient induces a kinetic asymmetry in the symport cycle of LacY.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  10. Isolation and structural characterization of a ZnThe Journal of biological chemistry · 2020
    Article
  11. Article
  12. It takes two to tango: The dance of the permease.The Journal of general physiology · 2019
    Review
  13. Mentors: Ron Kaback.The Journal of general physiology · 2019
    Article
  14. Engineered occluded apo-intermediate of LacY.Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  15. Article
  16. Article
  17. Article
  18. Crystal Structure of a ligand-bound LacY-Nanobody Complex.Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  19. The ins and outs of vesicular monoamine transporters.The Journal of general physiology · 2018
    Review
  20. Oversized galactosides as a probe for conformational dynamics in LacY.Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 4 countries.

Xin JiangState Key Laboratory of Membrane Biology, Center for Structural Biology, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China.
Irina SmirnovaDepartment of Physiology, University of California, Los Angeles, CA 90095.
Vladimir KashoDepartment of Physiology, University of California, Los Angeles, CA 90095.
Jianping WuState Key Laboratory of Membrane Biology, Center for Structural Biology, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China.
Kunio HirataAdvanced Photon Technology Division, Research Infrastructure Group, SR Life Science Instrumentation Unit, RIKEN/SPring-8 Center, Hyogo 679-5148, Japan.
Meng KeState Key Laboratory of Membrane Biology, Center for Structural Biology, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China.
Els PardonVIB Center for Structural Biology Research, VIB, 1050 Brussels, Belgium.
Jan SteyaertVIB Center for Structural Biology Research, VIB, 1050 Brussels, Belgium.
Nieng YanState Key Laboratory of Membrane Biology, Center for Structural Biology, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China.
H Ronald KabackDepartment of Physiology, University of California, Los Angeles, CA 90095; rkaback@mednet.ucla.edu.
Tsinghua University · CNUniversity of California, Los Angeles · USVrije Universiteit Brussel · BEJapan Science and Technology Agency · JP

Funding

Dynamics of the Lactose Permease of Escherichia ColiR01GM120043 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WRIGHT, ERNEST M · 2016 to 2019
$1.8M
NIGMS NIH HHS R01 GM120043
6 · The paper itself

Abstract

The lactose permease of Escherichia coli (LacY), a dynamic polytopic membrane protein, catalyzes galactoside-H

Indexed as

Protein ConformationBinding SitesCrystallography, X-RayEscherichia coli ProteinsModels, MolecularMonosaccharide Transport ProteinsMutationPeriplasmProtein BindingSingle-Domain AntibodiesSymportersEscherichia coli ProteinsLacY protein, E coliMonosaccharide Transport ProteinsSingle-Domain AntibodiesSymportersbioenergeticsfluorescencekineticsmembrane transporterX-ray structure

Identifiers

PMID27791182
PMCPMC5098631
OpenAlexW2532595958

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.