Evidence map›Paper›PMID 27791173›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2016

Prolactin alters blood pressure by modulating the activity of endothelial nitric oxide synthase.

Albert S Chang, Ruriko Grant, Hirofumi Tomita, Hyung-Suk Kim, Oliver Smithies, Masao Kakoki

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 1 pooled it
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.

  1. Evidence for hyperprolactinemia in migraineurs: a systematic review and meta-analysis.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2020
    Pooled it
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  9. The role of prolactin/vasoinhibins in cardiovascular diseases.Animal models and experimental medicine · 2023
    Review
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  11. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Albert S ChangDepartment of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599.
Ruriko GrantDepartment of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599.
Hirofumi TomitaDepartment of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599.
Hyung-Suk KimDepartment of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599.
Oliver SmithiesDepartment of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599 oliver_smithies@med.unc.edu mkakoki@med.unc.edu.
Masao KakokiDepartment of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599 oliver_smithies@med.unc.edu mkakoki@med.unc.edu.
University of North Carolina at Chapel Hill · US

Funding

MODELS FOR STUDYING THE GENETICS OF HYPERTENSIONR01HL049277 · NHLBI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI MAEDA, NOBUYO · 1992 to 2025
$17.5M
Duke-UNC-Stanford AMDC UnitU01HL070523 · NHLBI · DUKE UNIVERSITY · PI COFFMAN, THOMAS M · 2001 to 2005
$3.7M
Genetic Control of Protein Structure and SynthesisR01HL071266 · NHLBI · UNIVERSITY OF NORTH CAROLINA CHAPEL HILL · PI SMITHIES, OLIVER · 2002 to 2005
$1.5M
NHLBI NIH HHS R01 HL049277NHLBI NIH HHS R01 HL071266NHLBI NIH HHS U01 HL070523
6 · The paper itself

Abstract

Increased levels of a cleaved form of prolactin (molecular weight 16 kDa) have been associated with preeclampsia. To study the effects of prolactin on blood pressure (BP), we generated male mice with a single-copy transgene (Tg; inserted into the hypoxanthine-guanine phosphoribosyltransferase locus) that enables inducible hepatic production of prolactin and its cleavage product. The Tg is driven by the indole-3-carbinol (I3C)-inducible rat cytochrome P450 1A1 promoter. When the Tg mice were fed normal chow (NC), plasma prolactin concentrations were comparable to those in female WT mice in the last third of pregnancy, and BP was lower than in WT mice (∼95 mm Hg vs. ∼105 mm Hg). When the Tg mice were fed chow containing IC3, plasma prolactin concentrations increased threefold, BP increased to ∼130 mm Hg, and cardiac function became markedly impaired. IC3 chow did not affect the WT mice. Urinary excretion of nitrite/nitrate and the amount of Ser1177-phosphorylated endothelial nitric oxide (NO) synthase (eNOS) were significantly greater in the Tg mice fed NC than in WT mice, as they are during pregnancy. However, when I3C was fed, these indicators of NO production became significantly less in the Tg mice than in WT mice. The effects of increased plasma prolactin were abolished by a genetic absence of eNOS. Thus, a threefold increase in plasma prolactin is sufficient to increase BP significantly and to markedly impair cardiac function, with effects mediated by NO produced by eNOS. We suggest that pregnant women with abnormally high prolactin levels may need special attention.

Indexed as

Blood PressureAnimalsFemaleGene ExpressionHumansHypertensionIndolesMaleMice, KnockoutMice, TransgenicNitric OxideNitric Oxide Synthase Type IIIPhosphorylationPregnancyProlactinRatsindole-3-carbinolIndolesNitric OxideNitric Oxide Synthase Type IIIProlactinhypertensionlactationprotein kinase B/Akt

Identifiers

PMID27791173
PMCPMC5098649
OpenAlexW2538780408

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.