Evidence map›Paper›PMID 27791167›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2016

Super-resolution ribosome profiling reveals unannotated translation events in

Polly Yingshan Hsu, Lorenzo Calviello, Hsin-Yen Larry Wu, Fay-Wei Li, Carl J Rothfels, Uwe Ohler, Philip N Benfey

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 142 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
142citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

142 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Ribosome stalling position, spacing, and A-site occupancy impact translation and cotranslational mRNA decay in plants.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. CDS-localized mNature plants · 2026
    Article
  9. Article
  10. Article
  11. Maize Anther Development Involves Translated Open Reading Frames From 3' Untranslated Regions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  12. Article
  13. Article
  14. Article
  15. Translational landscape during seed germination revealed by ribosome profiling.The Plant journal : for cell and molecular biology · 2026
    Article
  16. Review
  17. Review
  18. Article
  19. TheGenome research · 2025
    Article
  20. Review

82 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Polly Yingshan HsuDepartment of Biology, Duke University, Durham, NC 27708.
Lorenzo CalvielloBerlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.
Hsin-Yen Larry WuBioinformatics Research Center and Department of Statistics, North Carolina State University, Raleigh, NC 27695.
Fay-Wei LiDepartment of Biology, Duke University, Durham, NC 27708.
Carl J RothfelsUniversity Herbarium, University of California, Berkeley, CA 94720.
Uwe OhlerBerlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.
Philip N BenfeyDepartment of Biology, Duke University, Durham, NC 27708; philip.benfey@duke.edu.

Funding

MOLECULAR GENETIC ANALYSIS OF ROOT MORPHOGENESISR01GM043778 · NIGMS · ROCKEFELLER UNIVERSITY · PI BENFEY, PHILIP N · 1991 to 2017
$5.4M
Genome Sequencer FLXS10RR029668 · NCRR · UNIVERSITY OF CALIFORNIA BERKELEY · PI NGAI, JOHN J. · 2010 to 2010
$623k
Illumina Sequencer to Facilitate Functional Genomics at BerkeleyS10RR027303 · NCRR · UNIVERSITY OF CALIFORNIA BERKELEY · PI TAYLOR, JOHN W. · 2010 to 2010
$500k
NCRR NIH HHS S10 RR027303NCRR NIH HHS S10 RR029668NIGMS NIH HHS R01 GM043778
6 · The paper itself

Abstract

Deep sequencing of ribosome footprints (ribosome profiling) maps and quantifies mRNA translation. Because ribosomes decode mRNA every 3 nt, the periodic property of ribosome footprints could be used to identify novel translated ORFs. However, due to the limited resolution of existing methods, the 3-nt periodicity is observed mostly in a global analysis, but not in individual transcripts. Here, we report a protocol applied to

Indexed as

ncRNARibo-seqribosome footprintsORFtranslation

Identifiers

PMID27791167
PMCPMC5111709

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.