ArticleProceedings of the National Academy of Sciences of the United States of America2016
Structural basis for dual specificity of yeast N-terminal amidase in the N-end rule pathway.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 36 citations in OpenAlex.
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- Genomic factors limiting the diversity of Saccharomycotina plant pathogens.G3 (Bethesda, Md.) · 2025Article
- Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recognin.Nature communications · 2025Article
- GID4 recognition of Pro/N-degron peptides: Conformational selection and induced fit.Biophysical journal · 2025Article
- N-degron pathways.Proceedings of the National Academy of Sciences of the United States of America · 2024Review
- Structural study for substrate recognition of human N-terminal glutamine amidohydrolase 1 in the arginine N-degron pathway.Protein science : a publication of the Protein Society · 2024Article
- Crystal structure of the Ate1 arginyl-tRNA-protein transferase and arginylation of N-degron substrates.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Aminopeptidases trim Xaa-Pro proteins, initiating their degradation by the Pro/N-degron pathway.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Arg/N-degron pathway targets transcription factors and regulates specific genes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The ATF3 Transcription Factor Is a Short-Lived Substrate of the Arg/N-Degron Pathway.Biochemistry · 2020Article
- Recognition of nonproline N-terminal residues by the Pro/N-degron pathway.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Five enzymes of the Arg/N-degron pathway form a targeting complex: The concept of superchanneling.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Use of the LC3B-fusion technique for biochemical and structural studies of proteins involved in the N-degron pathway.The Journal of biological chemistry · 2020Article
- Structural Analyses on the Deamidation of N-Terminal Asn in the Human N-Degron Pathway.Biomolecules · 2020Article
- Article
- Gid10 as an alternative N-recognin of the Pro/N-degron pathway.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- N-degron and C-degron pathways of protein degradation.Proceedings of the National Academy of Sciences of the United States of America · 2019Review
- Formyl-methionine as an N-degron of a eukaryotic N-end rule pathway.Science (New York, N.Y.) · 2018Article
- A reference-based protein degradation assay without global translation inhibitors.The Journal of biological chemistry · 2017Article
- Control of Hsp90 chaperone and its clients by N-terminal acetylation and the N-end rule pathway.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The first step of the hierarchically organized Arg/N-end rule pathway of protein degradation is deamidation of the N-terminal glutamine and asparagine residues of substrate proteins to glutamate and aspartate, respectively. These reactions are catalyzed by the N-terminal amidase (Nt-amidase) Nta1 in fungi such as Saccharomyces cerevisiae, and by the glutamine-specific Ntaq1 and asparagine-specific Ntan1 Nt-amidases in mammals. To investigate the dual specificity of yeast Nta1 (yNta1) and the importance of second-position residues in Asn/Gln-bearing N-terminal degradation signals (N-degrons), we determined crystal structures of yNta1 in the apo state and in complex with various N-degron peptides. Both an Asn-peptide and a Gln-peptide fit well into the hollow active site pocket of yNta1, with the catalytic triad located deeper inside the active site. Specific hydrogen bonds stabilize interactions between N-degron peptides and hydrophobic peripheral regions of the active site pocket. Key determinants for substrate recognition were identified and thereafter confirmed by using structure-based mutagenesis. We also measured affinities between yNta1 (wild-type and its mutants) and specific peptides, and determined K
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