ArticleProceedings of the National Academy of Sciences of the United States of America2016
Calcium transport into the cells of the sea urchin larva in relation to spicule formation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 80 citations in OpenAlex.
- Possible role of microtubules in vesicular transport of matrix protein during sea urchin larval biomineralization.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Proton channels govern vesicular carbonate chemistry in mineralizing cells of a marine calcifier.Nature communications · 2026Article
- The Role of Confinement in Biomineralization.Chemical reviews · 2025Review
- Dynamic change of calcium-rich compartments during coccolithophore biomineralization.Science advances · 2025Article
- A mechanosensitive circuit of FAK, ROCK, and ERK controls biomineral growth and morphology in the sea urchin embryo.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Exploring the Potential of Nonclassical Crystallization Pathways to Advance Cementitious Materials.Chemical reviews · 2024Review
- Mg-rich amorphous to Mg-low crystalline CaCOHeliyon · 2023Article
- Carbonic anhydrases in development: morphological observations and gene expression profiling in sea urchin embryos exposed to acetazolamide.Open biology · 2023Article
- Extracellular carbonic anhydrase activity promotes a carbon concentration mechanism in metazoan calcifying cells.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Distinct regulatory states control the elongation of individual skeletal rods in the sea urchin embryo.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Hydrocoel morphogenesis forming the pentaradial body plan in a sea cucumber, Apostichopus japonicus.Scientific reports · 2022Article
- Biomineralization: Integrating mechanism and evolutionary history.Science advances · 2022Review
- The Evolution of Biomineralization through the Co-Option of Organic Scaffold Forming Networks.Cells · 2022Review
- Ion Pathways in Biomineralization: Perspectives on Uptake, Transport, and Deposition of Calcium, Carbonate, and Phosphate.Journal of the American Chemical Society · 2021Article
- Article
- Rab35 regulates skeletogenesis and gastrulation by facilitating actin remodeling and vesicular trafficking.Cells & development · 2021Article
- Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization.PLoS computational biology · 2021Article
- Serial cryoFIB/SEM Reveals Cytoarchitectural Disruptions in Leigh Syndrome Patient Cells.Structure (London, England : 1993) · 2021Article
- Cellular pathways of calcium transport and concentration toward mineral formation in sea urchin larvae.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cellular bicarbonate accumulation and vesicular proton transport promote calcification in the sea urchin larva.Proceedings. Biological sciences · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the manner in which the sea urchin larva takes up calcium from its body cavity into the primary mesenchymal cells (PMCs) that are responsible for spicule formation. We used the membrane-impermeable fluorescent dye calcein and alexa-dextran, with or without a calcium channel inhibitor, and imaged the larvae in vivo with selective-plane illumination microscopy. Both fluorescent molecules are taken up from the body cavity into the PMCs and ectoderm cells, where the two labels are predominantly colocalized in particles, whereas the calcium-binding calcein label is mainly excluded from the endoderm and is concentrated in the spicules. The presence of vesicles and vacuoles inside the PMCs that have openings through the plasma membrane directly to the body cavity was documented using high-resolution cryo-focused ion beam-SEM serial imaging. Some of the vesicles and vacuoles are interconnected to form large networks. We suggest that these vacuolar networks are involved in direct sea water uptake. We conclude that the calcium pathway from the body cavity into cells involves nonspecific endocytosis of sea water with its calcium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.