ArticleProceedings of the National Academy of Sciences of the United States of America2016
Laminin targeting of a peripheral nerve-highlighting peptide enables degenerated nerve visualization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Intraoperative near-infrared fluorescence imaging can identify pelvic nerves in patients with cervical cancer in real time during radical hysterectomy.European journal of nuclear medicine and molecular imaging · 2022Trial
- Mechanisms and Applications of Photocatalytic Proximity Labeling.Chemical & biomedical imaging · 2026Review
- A nanozyme-augmented peripheral nerve-targeting liposome alleviates local immune inflammation and ferroptosis for enhanced facial nerve repair.Materials today. Bio · 2026Article
- The Usefulness of Indocyanine Green in Modern Gynecological Oncology-Analysis, Literature Review, and Future Perspectives.Cancers · 2025Review
- µMap proximity labeling in living cells reveals stress granule disassembly mechanisms.Nature chemical biology · 2025Article
- Understanding the molecular diversity of synapses.Nature reviews. Neuroscience · 2025Review
- Photoproximity labeling of endogenous receptors in the live mouse brain in minutes.Nature chemical biology · 2025Article
- NIR-II light in clinical oncology: opportunities and challenges.Nature reviews. Clinical oncology · 2024Review
- Realizing real-time optical molecular imaging in peripheral nerve tissue via Rhodamine B.Frontiers in medicine · 2024Article
- [Near-infrared targeted probe designed for intraoperative imaging of prostatic neurovascular bundles].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2023Article
- Advances in optical molecular imaging for neural visualization.Frontiers in bioengineering and biotechnology · 2023Review
- Proximity-Dependent Biotinylation Approaches to Explore the Dynamic Compartmentalized Proteome.Frontiers in molecular biosciences · 2022Review
- Contact-ID, a tool for profiling organelle contact sites, reveals regulatory proteins of mitochondrial-associated membrane formation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Sight and switch off: Nerve density visualization for interventions targeting nerves in prostate cancer.Science advances · 2020Article
- Systemically Administered, Target-Specific, Multi-Functional Therapeutic Recombinant Proteins in Regenerative Medicine.Nanomaterials (Basel, Switzerland) · 2020Review
- Imaging in the repair of peripheral nerve injury.Nanomedicine (London, England) · 2019Article
- Glycomics and Proteomics Approaches to Investigate Early Adenovirus-Host Cell Interactions.Journal of molecular biology · 2018Review
- Nerve-targeted probes for fluorescence-guided intraoperative imaging.Theranostics · 2018Article
- The Effect of Muscle Graft With Nerve Growth Factor and Laminin on Sciatic Nerve Repair in Rats.Basic and clinical neuroscienceArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
Target-blind activity-based screening of molecular libraries is often used to develop first-generation compounds, but subsequent target identification is rate-limiting to developing improved agents with higher specific affinity and lower off-target binding. A fluorescently labeled nerve-binding peptide, NP41, selected by phage display, highlights peripheral nerves in vivo. Nerve highlighting has the potential to improve surgical outcomes by facilitating intraoperative nerve identification, reducing accidental nerve transection, and facilitating repair of damaged nerves. To enable screening of molecular target-specific molecules for higher nerve contrast and to identify potential toxicities, NP41's binding target was sought. Laminin-421 and -211 were identified by proximity-based labeling using singlet oxygen and by an adapted version of TRICEPS-based ligand-receptor capture to identify glycoprotein receptors via ligand cross-linking. In proximity labeling, photooxidation of a ligand-conjugated singlet oxygen generator is coupled to chemical labeling of locally oxidized residues. Photooxidation of methylene blue-NP41-bound nerves, followed by biotin hydrazide labeling and purification, resulted in light-induced enrichment of laminin subunits α4 and α2, nidogen 1, and decorin (FDR-adjusted
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.