ArticleProceedings of the National Academy of Sciences of the United States of America2016
Kinetics of DNA uptake during transformation provide evidence for a translocation ratchet mechanism.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 62 citations in OpenAlex.
- The Trajectory Statistics of Biological Exploratory Dynamics.bioRxiv : the preprint server for biology · 2026Article
- Molecular interplay between ComEC domains allows for selective degradation of the non-translocating strand during natural transformation.Nucleic acids research · 2025Article
- Naturally competent bacteria and their genetic parasites-a battle for control over horizontal gene transfer?FEMS microbiology reviews · 2025Review
- Pilin antigenic variants impact gonococcal lifestyle and antibiotic tolerance by modulating interbacterial forces.PLoS biology · 2025Article
- Article
- DNA Transport through the Dynamic Type IV Secretion System.Infection and immunity · 2023Review
- Genetic manipulation of candidate phyla radiation bacteria provides functional insights into microbial dark matter.bioRxiv : the preprint server for biology · 2023Article
- Cj0683 Is a Competence Protein Essential for Efficient Initialization of DNA Uptake inBiomolecules · 2023Article
- Multisite transformation inFrontiers in microbiology · 2023Article
- Structure-function studies reveal ComEA contains an oligomerization domain essential for transformation in gram-positive bacteria.Nature communications · 2022Article
- Article
- Experimental study of a nanoscale translocation ratchet.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Hydrodynamic synchronization and clustering in ratcheting colloidal matter.Science advances · 2022Article
- The molecular basis of FimT-mediated DNA uptake during bacterial natural transformation.Nature communications · 2022Article
- Kingella kingae PilC1 and PilC2 are adhesive multifunctional proteins that promote bacterial adherence, twitching motility, DNA transformation, and pilus biogenesis.PLoS pathogens · 2022Article
- Bacterial motility: machinery and mechanisms.Nature reviews. Microbiology · 2022Review
- The molecular diversity of transcriptional factor TfoX is a determinant in natural transformation inFrontiers in microbiology · 2022Article
- Article
- Article
- Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation.Journal of bacteriology · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Horizontal gene transfer can speed up adaptive evolution and support chromosomal DNA repair. A particularly widespread mechanism of gene transfer is transformation. The initial step to transformation, namely the uptake of DNA from the environment, is supported by the type IV pilus system in most species. However, the molecular mechanism of DNA uptake remains elusive. Here, we used single-molecule techniques for characterizing the force-dependent velocity of DNA uptake by Neisseria gonorrhoeae We found that the DNA uptake velocity depends on the concentration of the periplasmic DNA-binding protein ComE, indicating that ComE is directly involved in the uptake process. The velocity-force relation of DNA uptake is in very good agreement with a translocation ratchet model where binding of chaperones in the periplasm biases DNA diffusion through a membrane pore in the direction of uptake. The model yields a speed of DNA uptake of 900 bp⋅s
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