ArticleProceedings of the National Academy of Sciences of the United States of America2016
Scaffoldless engineered enzyme assembly for enhanced methanol utilization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 127 citations in OpenAlex.
- Advances in synthetic biology for engineering methylotrophic microbial cell factories.Journal of bacteriology · 2026Review
- Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy.Nature communications · 2025Article
- Centromeric Sequences in Ogataea polymorpha Genome Enable Development of Stable Multigene Expression Plasmid Tools.Microbial biotechnology · 2025Article
- Sustainable Bioconversion of Methanol: A Renewable Employing Novel Alcohol Oxidase and Pyruvate Aldolase.Journal of agricultural and food chemistry · 2025Article
- Bringing carbon to life via one-carbon metabolism.Trends in biotechnology · 2025Review
- Seven critical challenges in synthetic one-carbon assimilation and their potential solutions.FEMS microbiology reviews · 2025Review
- Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives.Synthetic and systems biotechnology · 2023Review
- Perspectives for Using COBioengineering (Basel, Switzerland) · 2023Review
- Metabolic engineering strategies for microbial utilization of methanol.Engineering microbiology · 2023Review
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- Application of artificial scaffold systems in microbial metabolic engineering.Frontiers in bioengineering and biotechnology · 2023Review
- Metabolic pathway assembly using docking domains from type I cis-AT polyketide synthases.Nature communications · 2022Article
- Biosynthesis of Linear Protein Nanoarrays Using the Flagellar Axoneme.ACS synthetic biology · 2022Article
- Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.Microorganisms · 2022Review
- From a Hetero- to a Methylotrophic Lifestyle: Flash Back on the Engineering Strategies to Create Synthetic Methanol-User Strains.Frontiers in bioengineering and biotechnology · 2022Review
- Bioconversion of Methanol by Synthetic Methylotrophy.Advances in biochemical engineering/biotechnology · 2022Article
- Strategies for Multienzyme Assemblies.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Metabolic engineering strategies to enable microbial utilization of C1 feedstocks.Nature chemical biology · 2021Review
- Transcriptome analysis reveals the roles of nitrogen metabolism and sedoheptulose bisphosphatase pathway in methanol-dependent growth of Corynebacterium glutamicum.Microbial biotechnology · 2021Article
- Methanol Dehydrogenases as a Key Biocatalysts for Synthetic Methylotrophy.Frontiers in bioengineering and biotechnology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methanol is an important feedstock derived from natural gas and can be chemically converted into commodity and specialty chemicals at high pressure and temperature. Although biological conversion of methanol can proceed at ambient conditions, there is a dearth of engineered microorganisms that use methanol to produce metabolites. In nature, methanol dehydrogenase (Mdh), which converts methanol to formaldehyde, highly favors the reverse reaction. Thus, efficient coupling with the irreversible sequestration of formaldehyde by 3-hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloseisomerase (Phi) serves as the key driving force to pull the pathway equilibrium toward central metabolism. An emerging strategy to promote efficient substrate channeling is to spatially organize pathway enzymes in an engineered assembly to provide kinetic driving forces that promote carbon flux in a desirable direction. Here, we report a scaffoldless, self-assembly strategy to organize Mdh, Hps, and Phi into an engineered supramolecular enzyme complex using an SH3-ligand interaction pair, which enhances methanol conversion to fructose-6-phosphate (F6P). To increase methanol consumption, an "NADH Sink" was created using
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.