ArticleProceedings of the National Academy of Sciences of the United States of America2016
Terpene synthase genes in eukaryotes beyond plants and fungi: Occurrence in social amoebae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 106 citations in OpenAlex.
- Early cAMP signaling orchestrates single-cell synchronicity throughout Dictyostelium development.Communications biology · 2026Article
- Global profiling of polyketide synthases in facultative multicellular eukaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mechanistic Characterisation of a Diterpene Synthase for Chryseojoostenes A-E from Chryseobacterium Joostei.Angewandte Chemie (International ed. in English) · 2025Article
- Structural insights into a bacterial terpene cyclase fused with haloacid Dehalogenase-like phosphatase.Chemical science · 2025Article
- Identification of Slime Mold Metabolites That Confer Protection to Commercial Crops against Root-Knot Nematodes.Journal of agricultural and food chemistry · 2025Article
- Exploring the microbial communities in coastal cenote and their hidden biotechnological potential.Microbial genomics · 2025Article
- The Amoeba Dictyostelium discoideum as Novel Production Host for Complex Substances.Advances in biochemical engineering/biotechnology · 2025Review
- Canonical terpene synthases in arthropods: Intraphylum gene transfer.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Functional Redundancy and Dual Function of a Hypothetical Protein in the Biosynthesis of Eunicellane-Type Diterpenoids.ACS chemical biology · 2024Article
- Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket.Synthetic and systems biotechnology · 2024Article
- A diterpene synthase from the sandflyProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Stability trends in carbocation intermediates stemming from germacrene A and hedycaryol.Beilstein journal of organic chemistry · 2024Article
- Role of Microbial Volatile Organic Compounds in Promoting Plant Growth and Disease Resistance in Horticultural Production.Plant signaling & behavior · 2023Review
- Molecular and Functional Analyses of Characterized Sesquiterpene Synthases in Mushroom-Forming Fungi.Journal of fungi (Basel, Switzerland) · 2023Article
- Article
- Expression of terpene synthase-related genes in parents and offspring ofFood chemistry. Molecular sciences · 2023Article
- Functional characterisation of twelve terpene synthases from actinobacteria.Beilstein journal of organic chemistry · 2023Article
- Yellow polyketide pigment suppresses premature hatching in social amoeba.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A terpene cyclase fromRSC advances · 2022Article
- Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
Terpenes are structurally diverse natural products involved in many ecological interactions. The pivotal enzymes for terpene biosynthesis, terpene synthases (TPSs), had been described only in plants and fungi in the eukaryotic domain. In this report, we systematically analyzed the genome sequences of a broad range of nonplant/nonfungus eukaryotes and identified putative TPS genes in six species of amoebae, five of which are multicellular social amoebae from the order of Dictyosteliida. A phylogenetic analysis revealed that amoebal TPSs are evolutionarily more closely related to fungal TPSs than to bacterial TPSs. The social amoeba Dictyostelium discoideum was selected for functional study of the identified TPSs. D. discoideum grows as a unicellular organism when food is abundant and switches from vegetative growth to multicellular development upon starvation. We found that expression of most D. discoideum TPS genes was induced during development. Upon heterologous expression, all nine TPSs from D. discoideum showed sesquiterpene synthase activities. Some also exhibited monoterpene and/or diterpene synthase activities. Direct measurement of volatile terpenes in cultures of D. discoideum revealed essentially no emission at an early stage of development. In contrast, a bouquet of terpenes, dominated by sesquiterpenes including β-barbatene and (E,E)-α-farnesene, was detected at the middle and late stages of development, suggesting a development-specific function of volatile terpenes in D. discoideum. The patchy distribution of TPS genes in the eukaryotic domain and the evidence for TPS function in D. discoideum indicate that the TPS genes mediate lineage-specific adaptations.
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