ArticleMicrobiologyOpen2017
One step forwards for the routine use of high-throughput DNA sequencing in environmental monitoring. An efficient and standardizable method to maximize the detection of environmental bacteria.
Article in MicrobiologyOpen, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Conservation Planning and Reporting Implications of qPCR-based Multi-species eDNA Detection Under EU Environmental Regulations.Environmental management · 2026Article
- Deciphering the fungal symphony: unveiling the fungal dynamics during the fermentation of traditional Chinese strong-flavorFrontiers in microbiology · 2025Article
- Aquaculture ecosystem microbiome at the water-fish interface: the case-study of rainbow trout fed with Tenebrio molitor novel diets.BMC microbiology · 2023Article
- Capturing marine microbiomes and environmental DNA: A field sampling guide.Frontiers in microbiology · 2022Review
- The Requirement of Genetic Diagnostic Technologies for Environmental Surveillance of Antimicrobial Resistance.Sensors (Basel, Switzerland) · 2021Article
- Characterization of bacterial community and flavor differences of different types of Douchi.Food science & nutrition · 2021Article
- Analysis of microbial diversity and functional differences in different types of high-temperature Daqu.Food science & nutrition · 2021Article
- Exploring Changes in the Microbiota ofFrontiers in microbiology · 2021Article
- Improving eDNA yield and inhibitor reduction through increased water volumes and multi-filter isolation techniques.Scientific reports · 2019Article
- Article
- A metagenomic survey of soil microbial communities along a rehabilitation chronosequence after iron ore mining.Scientific data · 2019Article
- Review
- Changes in the Drinking Water Microbiome: Effects of Water Treatments Along the Flow of Two Drinking Water Treatment Plants in a Urbanized Area, Milan (Italy).Frontiers in microbiology · 2018Article
- Geographical and Cultivar Features Differentiate Grape Microbiota in Northern Italy and Spain Vineyards.Frontiers in microbiology · 2018Article
- Exploring the under-investigated "microbial dark matter" of drinking water treatment plants.Scientific reports · 2017Article
- One step forwards for the routine use of high-throughput DNA sequencing in environmental monitoring. An efficient and standardizable method to maximize the detection of environmental bacteria.MicrobiologyOpen · 2017Article
- Grape microbiome as a reliable and persistent signature of field origin and environmental conditions in Cannonau wine production.PloS one · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We propose an innovative, repeatable, and reliable experimental workflow to concentrate and detect environmental bacteria in drinking water using molecular techniques. We first concentrated bacteria in water samples using tangential flow filtration and then we evaluated two methods of environmental DNA extraction. We performed tests on both artificially contaminated water samples and real drinking water samples. The efficiency of the experimental workflow was measured through qPCR. The successful applicability of the high-throughput DNA sequencing (HTS) approach was demonstrated on drinking water samples. Our results demonstrate the feasibility of our approach in high-throughput-based studies, and we suggest incorporating it in monitoring strategies to have a better representation of the microbial community. In the recent years, HTS techniques have become key tools in the study of microbial communities. To make the leap from academic laboratories to the routine monitoring (e.g., water treatment plants laboratories), we here propose an experimental workflow suitable for the introduction of HTS as a standard method for detecting environmental bacteria.
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Registered trials
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