Evidence map›Paper›PMID 27790194›Full record

ArticleFrontiers in microbiology2016

A Sample-to-Sequence Protocol for Genus Targeted Transcriptomic Profiling: Application to Marine

Frances D Pitt, Andrew Millard, Martin Ostrowski, Suat Dervish, Sophie Mazard, Ian T Paulsen, Mikhail V Zubkov, David J Scanlan

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. A distinct, high-affinity, alkaline phosphatase facilitates occupation of P-depleted environments by marine picocyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Frances D PittSchool of Life Sciences, University of Warwick Coventry, UK.
Andrew MillardWarwick Medical School, University of Warwick Coventry, UK.
Martin OstrowskiDepartment of Biochemistry and Biomolecular Science, Faculty of Science and Engineering, Macquarie University Sydney, NSW, Australia.
Suat DervishSydney Cytometry, Centenary Institute, University of Sydney Sydney, NSW, Australia.
Sophie MazardDepartment of Biochemistry and Biomolecular Science, Faculty of Science and Engineering, Macquarie University Sydney, NSW, Australia.
Ian T PaulsenDepartment of Biochemistry and Biomolecular Science, Faculty of Science and Engineering, Macquarie University Sydney, NSW, Australia.
Mikhail V ZubkovNational Oceanography Centre Southampton, UK.
David J ScanlanSchool of Life Sciences, University of Warwick Coventry, UK.
Macquarie University · AUUniversity of Warwick · GBCentenary Institute · AUNational Oceanography Centre · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent studies using whole community metagenomic and metatranscriptomic approaches are revealing important new insights into the functional potential and activity of natural marine microbial communities. Here, we complement these approaches by describing a complete ocean sample-to-sequence protocol, specifically designed to target a single bacterial genus for purposes of both DNA and RNA profiling using fluorescence activated cell sorting (FACS). The importance of defining and understanding the effects of a sampling protocol are critical if we are to gain meaningful data from environmental surveys. Rigorous pipeline trials with a cultured isolate,

Indexed as

flow cytometryfluorescence activated cell sortingmarine microbiologyRNASynechococcustranscriptomics

Identifiers

PMID27790194
PMCPMC5063861
OpenAlexW2530631626

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.