ArticleActa stomatologica Croatica2016
Comparison of RNA Extraction Methods for Molecular Analysis of Oral Cytology.
Article in Acta stomatologica Croatica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 17 citations in OpenAlex.
- Assessing the impact of storage conditions on RNA from human saliva and its application to the identification of mRNA biomarkers for asthma.Frontiers in molecular biosciences · 2024Article
- Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus.Advances in virology · 2023Article
- Transcriptome analysis of intestine from alk-SMase knockout mice reveals the effect of alk-SMase.Cancer cell international · 2022Article
- Optimization of pre-analytical and analytical steps for DNA and RNA analysis of fresh cytology samples.Cancer medicine · 2022Article
- Transcriptome analysis alk-SMase knockout mice reveals the effect of alkaline sphingomyelinase on liver.Biochemistry and biophysics reports · 2022Article
- Effects of Shear Stress on Production of FVIII and vWF in a Cell-Based Therapeutic for Hemophilia A.Frontiers in bioengineering and biotechnology · 2021Article
- Improvement in RNA quantity and quality in cervico-vaginal cytology.Virology journal · 2020Article
- Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice.Journal of visualized experiments : JoVE · 2019Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objective of workThe aim of this study was to compare three methods of RNA extraction for molecular analysis of oral cytology to establish the best technique, considering its concentration and purity for molecular tests of oral lesions such as real-time reverse transcriptase reaction. MATERIAL AND
methodsThe sample included exfoliative cytology from the oral cavity mucosa of patients with no visible clinical changes, using Orcellex Rovers Brush
resultsTrizol® group revealed higher average concentration, followed by Direct-zol
conclusionConsidering all aspects, concentration, purity and time spent in the procedures, the Direct-zol
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Registered trials
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