ArticleJournal of experimental botany2016
Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Intra-Relations of Biochemical Profiles Defining the Germination Potential of Medium-Term Cold-Stored Maize (Plants (Basel, Switzerland) · 2026Article
- Structural, functional and proteomic changes of proteins during rice yellowing.Food chemistry: X · 2025Article
- Large and stable genome edits at the sorghum alpha kafirin locus result in changes in chromatin accessibility and globally increased expression of genes encoding lysine enrichment.Frontiers in plant science · 2023Article
- Generation of multi-layered protein bodies inFrontiers in plant science · 2023Article
- The molecular basis of cereal grain proteostasis.Essays in biochemistry · 2022Review
- Identification of closely associated SNPs and candidate genes with seed size and shape via deep re-sequencing GWAS in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Specialized endoplasmic reticulum-derived vesicles in plants: Functional diversity, evolution, and biotechnological exploitation.Journal of integrative plant biology · 2022Review
- Wheat α-gliadin and high-molecular-weight glutenin subunit accumulate in different storage compartments of transgenic soybean seed.Transgenic research · 2022Article
- Microautophagy Mediates Vacuolar Delivery of Storage Proteins in Maize Aleurone Cells.Frontiers in plant science · 2022Article
- Integrative transcriptomic and TMT-based proteomic analysis reveals the mechanism by whichFrontiers in plant science · 2022Article
- SWATH-MS based quantitive proteomics reveal regulatory metabolism and networks of androdioecy breeding system in Osmanthus fragrans.BMC plant biology · 2021Article
- The regulation of zein biosynthesis in maize endosperm.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Towards coeliac-safe bread.Plant biotechnology journal · 2020Article
- Label-Free Comparative Proteomic Analysis Combined with Laser-Capture Microdissection Suggests Important Roles of Stress Responses in the Black Layer of Maize Kernels.International journal of molecular sciences · 2020Article
- Comprehensive comparison and applications of different sections in investigating the microstructure and histochemistry of cereal kernels.Plant methods · 2020Review
- Updating and interaction of polycomb repressive complex 2 components in maize (Zea mays).Planta · 2019Article
- Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour.BMC plant biology · 2019Article
- Myosin-driven transport network in plants.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prolamins, the major cereal seed storage proteins, are sequestered and accumulated in the lumen of the endoplasmic reticulum (ER), and are directly assembled into protein bodies (PBs). The content and composition of prolamins are the key determinants for protein quality and texture-related traits of the grain. Concomitantly, the PB-inducing fusion system provides an efficient target to produce therapeutic and industrial products in plants. However, the proteome of the native PB and the detailed mechanisms underlying its formation still need to be determined. We developed a method to isolate highly purified and intact PBs from developing maize endosperm and conducted proteomic analysis of intact PBs of zein, a class of prolamine protein found in maize. We thus identified 1756 proteins, which fall into five major categories: metabolic pathways, response to stimulus, transport, development, and growth, as well as regulation. By comparing the proteomes of crude and enriched extractions of PBs, we found substantial evidence for the following conclusions: (i) ribosomes, ER membranes, and the cytoskeleton are tightly associated with zein PBs, which form the peripheral border; (ii) zein RNAs are probably transported and localized to the PB-ER subdomain; and (iii) ER chaperones are essential for zein folding, quality control, and assembly into PBs. We futher confirmed that OPAQUE1 (O1) cannot directly interact with FLOURY1 (FL1) in yeast, suggesting that the interaction between myosins XI and DUF593-containing proteins is isoform-specific. This study provides a proteomic roadmap for dissecting zein PB biogenesis and reveals an unexpected diversity and complexity of proteins in PBs.
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