ArticleZebrafish2017
A Low-Cost Method of Skin Swabbing for the Collection of DNA Samples from Small Laboratory Fish.
Article in Zebrafish, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Diversity, antifungal susceptibility, and biofilm formation of fish-associated yeasts from freshwater and marine fish.Veterinary research communications · 2026Article
- Molecular characterization of FAM222B as a novel disease gene for dominant cardiovascular laterality defects.Scientific reports · 2026Article
- Protocol for non-invasive genotyping of axolotls using skin swabbing.STAR protocols · 2026Article
- Comparison of whole genome sequencing performance from fish swabs and fin clips.BMC research notes · 2025Article
- A RepetitiveInternational journal of molecular sciences · 2024Article
- The metalloproteinase PAPP-A is required for IGF-dependent chondrocyte differentiation and organization.Scientific reports · 2024Article
- Pain management in zebrafish : Report from a FELASA Working Group.Laboratory animals · 2024Review
- Whole-genome Comparisons Identify Repeated Regulatory Changes Underlying Convergent Appendage Evolution in Diverse Fish Lineages.Molecular biology and evolution · 2023Article
- Non-Lethal Detection ofViruses · 2023Article
- Whole-genome comparisons identify repeated regulatory changes underlying convergent appendage evolution in diverse fish lineages.bioRxiv : the preprint server for biology · 2023Article
- Quality of DNA extracted from freshwater fish scales and mucus and its application in genetic diversity studies ofBiology methods & protocols · 2023Article
- A minimally invasive fin scratching protocol for fast genotyping and early selection of zebrafish embryos.Scientific reports · 2022Article
- Refinement in the European Union: A Systematic Review.Animals : an open access journal from MDPI · 2022Review
- A Rapid and Noninvasive Method That Extracts Polymerase Chain Reaction-Ready Genomic DNA from Adult Zebrafish.Zebrafish · 2022Article
- Skin bacterial microbiome diversity predicts lower activity levels in female, but not male, guppies,Biology letters · 2022Article
- Article
- Longer or shorter spines: Reciprocal trait evolution in stickleback via triallelic regulatory changes inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- A Preliminary Assessment of Skin Microbiome Diversity of Zebrafish (Indian journal of microbiology · 2021Article
- Skin swabbing is a refined technique to collect DNA from model fish species.Scientific reports · 2020Article
- Modeling Neuronal Diseases in Zebrafish in the Era of CRISPR.Current neuropharmacology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Fin clipping of live fish under anesthesia is widely used to collect samples for DNA extraction. An alternative, potentially less invasive, approach involves obtaining samples by swabbing the skin of nonanesthetized fish. However, this method has yet to be widely adopted for use in laboratory studies in the biological and biomedical sciences. Here, we compare DNA samples from zebrafish Danio rerio and three-spined sticklebacks Gasterosteus aculeatus collected via fin clipping and skin swabbing techniques, and test a range of DNA extraction methods, including commercially available kits and a lower-cost, in-house method. We verify the method for polymerase chain reaction analysis, and examine the potential risk of cross contamination between individual fish that are netted together. We show that swabbing, which may not require the use of anesthesia or analgesics, offers a reliable alternative to fin clipping. Further work is now required to determine the relative effects of fin clipping and swabbing on the stress responses and subsequent health of fish, and hence the potential of swabbing as a refinement to existing DNA sampling procedures.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.