ArticleMethods in molecular biology (Clifton, N.J.)2019
The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine.
Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Cultivating the endogenous life cycle ofParasitology · 2025Article
- The Chicken Embryo: An Alternative Animal Model in Development, Disease and Pharmacological Treatment.Pharmacology research & perspectives · 2025Review
- Chicken-derived RSPO1 and WNT3 contribute to maintaining longevity of chicken intestinal organoid cultures.Scientific reports · 2022Article
- Avian Cell Culture Models to Study Immunomodulatory Properties of Bioactive Products.Animals : an open access journal from MDPI · 2022Review
- Farm and Companion Animal Organoid Models in Translational Research: A Powerful Tool to Bridge the Gap Between Mice and Humans.Frontiers in medical technology · 2022Review
- Derivation of snake venom gland organoids for in vitro venom production.Nature protocols · 2021Article
- Intestinal organoids in farm animals.Veterinary research · 2021Review
- Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis.Frontiers in cell and developmental biology · 2021Review
- Production and characterization of avian crypt-villus enteroids and the effect of chemicals.BMC veterinary research · 2020Article
- The migration and fusion events related to ROCK activity strongly influence the morphology of chicken embryo intestinal organoids.Protoplasma · 2019Article
- The formation of intestinal organoids in a hanging drop culture.Cytotechnology · 2018Article
- Recent Advances in Intestinal Stem Cells.Current molecular biology reports · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intestinal epithelium isolated from chicken embryos in last 3 days of development can be used to establish the 3D culture of intestinal organoids. When fragments of epithelial tissue released by incubation with EGTA (2.5 mM, 2 h) are embedded in Matrigel matrix on cell culture inserts the formation of empty spheres covered by epithelial cells is observed in first 24 h of culture. The growth and survival of organoids are supported by the addition of R-spondin 1, Noggin, and prostaglandin E
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.