ArticleCurrent microbiology2017
Engineering Plasmid-Free Klebsiella Pneumoniae for Production of 3-Hydroxypropionic Acid.
Article in Current microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 11 citations in OpenAlex.
- Establishing aMolecules (Basel, Switzerland) · 2022Article
- Detection of a New Resistance-Mediating Plasmid Chimera in aMicroorganisms · 2021Article
- Production of 3-Hydroxypropanoic Acid From Glycerol by Metabolically Engineered Bacteria.Frontiers in bioengineering and biotechnology · 2019Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although plasmid-dependent microbial breeding is predominant in manufacturing bio-based chemicals, it shows pitfall of genetic instability and thus hinders industrial production. Alternatively, chromosome engineering free from plasmid enables genetic stability and thus represents the new trend of microbial breeding. 3-Hydroxypropionic acid (3-HP) is an economically important platform compound as the versatile precursor of a suite of C3 compound, such as 1, 3-PDO. Klebsiella pneumoniae is regarded as a promising host strain due to both its dha regulon and exceptional glycerol fermentation ability. To produce 3-HP in K. pneumoniae, the IS1 region in chromosome was replaced with the AD DNA cassette containing aldH gene from E.coli through homologous recombination approach. The engineered recombinant converted glycerol into 3-HPA and then 3-HP when 40 g/L of initial glycerol was added. The novelties of this study comprise (i) the genetic stability of plasmid-free strains (ii) without using any inducer and antibiotics and thus more applicable than plasmid-based strains.
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