ArticleEpigenetics2017
De novo methylation in male germ cells of the common marmoset monkey occurs during postnatal development and is maintained in vitro.
Article in Epigenetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Establishment of DNA methylation during primate germ cell development.Nature communications · 2026Article
- New perspectives on DNA methylation modifications in ocular diseases.International journal of ophthalmology · 2025Review
- Genome-wide DNA methylation changes in human spermatogenesis.American journal of human genetics · 2024Article
- mRNA-based generation of marmoset PGCLCs capable of differentiation into gonocyte-like cells.Stem cell reports · 2023Article
- Cryopreservation of Ovarian and Testicular Tissue and the Influence on Epigenetic Pattern.International journal of molecular sciences · 2023Review
- Inheritance of paternal lifestyles and exposures through sperm DNA methylation.Nature reviews. Urology · 2023Review
- Sperm DNA methylation is predominantly stable in mice offspring born after transplantation of long-term cultured spermatogonial stem cells.Clinical epigenetics · 2023Article
- An Interplay between Epigenetics and Translation in Oocyte Maturation and Embryo Development: Assisted Reproduction Perspective.Biomedicines · 2022Review
- Short-Term Hypothermic Holding of Mouse Immature Testicular Tissue Does Not Alter the Expression of DNA Methyltransferases and Global DNA Methylation Level, Post-Organotypic Culture.Frontiers in endocrinology · 2022Article
- Whole-genome methylation analysis of testicular germ cells from cryptozoospermic men points to recurrent and functionally relevant DNA methylation changes.Clinical epigenetics · 2021Article
- The sperm epigenome does not display recurrent epimutations in patients with severely impaired spermatogenesis.Clinical epigenetics · 2020Article
- Development and Disease-Dependent Dynamics of Spermatogonial Subpopulations in Human Testicular Tissues.Journal of clinical medicine · 2020Article
- Fertility preservation in boys: recent developments and new insightsHuman reproduction open · 2020Article
- Article
- High-resolution analysis of germ cells from men with sex chromosomal aneuploidies reveals normal transcriptome but impaired imprinting.Clinical epigenetics · 2019Article
- An Epigenome-Wide DNA Methylation Map of Testis in Pigs for Study of Complex Traits.Frontiers in genetics · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The timing of de novo DNA methylation in male germ cells during human testicular development is yet unsolved. Apart from that, the stability of established imprinting patterns in vitro is controversially discussed. This study aimed at determining the timing of DNA de novo methylation and at assessing the stability of the methylation status in vitro. We employed the marmoset monkey (Callithrix jacchus) as it is considered the best non-human primate model for human testicular development. We selected neonatal, pre-pubertal, pubertal, and adult animals (n = 3, each) and assessed germ cell global DNA methylation levels by 5-methyl cytosine staining, and Alu elements and gene-specific methylation (H19, LIT1, SNRPN, MEST, OCT4, MAGE-A4, and DDX-4) by pyrosequencing. De novo methylation is progressively established during postnatal primate development and continues until adulthood, a process that is different in most other species. Importantly, once established, methylation patterns remained stable, as demonstrated using in vitro cultures. Thus, the marmoset monkey is a unique model for the study of postnatal DNA methylation mechanisms in germ cells and for the identification of epimutations and their causes.
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