Evidence map›Paper›PMID 27786294›Full record

ArticleScientific reports2016

Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus.

YuDong Liu, Li Zhang, LiJing Chen, Hui Ma, YanYe Ruan, Tao Xu, ChuanQiang Xu, Yi He, MingFang Qi

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
4.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. AnDHN, a Dehydrin Protein FromFrontiers in plant science · 2021
    Article
  6. Review
  7. Expression of Two α-Type Expansins fromInternational journal of molecular sciences · 2019
    Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

YuDong LiuHorticulture Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
Li ZhangKey Laboratory of Agricultural Biotechnology of Liaoning Province, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
LiJing ChenKey Laboratory of Agricultural Biotechnology of Liaoning Province, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
Hui MaKey Laboratory of Agricultural Biotechnology of Liaoning Province, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
YanYe RuanLiaoning Plant Gene Engineering Research Center, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
Tao XuHorticulture Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
ChuanQiang XuHorticulture Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
Yi HeHorticulture Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
MingFang QiHorticulture Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
Liaoning Academy of Agricultural Sciences · CNMinistry of Education of the People's Republic of China · CNShenyang Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Based on the galactinol synthase (AnGolS1) fragment sequence from a cold-induced Suppression Subtractive Hybridization (SSH) library derived from Ammopiptanthus nanus (A. nanus) seedlings, AnGolS1 mRNA (including the 5' UTR and 3' UTR) (GenBank accession number: GU942748) was isolated and characterized by rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). A substrate reaction test revealed that AnGolS1 possessed galactinol synthase activity in vitro and could potentially be an early-responsive gene. Furthermore, quantitative real-time PCR (qRT-PCR) indicated that AnGolS1 was responded to cold, salts and drought stresses, however, significantly up-regulated in all origans by low temperatures, especially in plant stems. In addition, the hybridization signals in the fascicular cambium were strongest in all cells under low temperature. Thus, we propose that AnGolS1 plays critical roles in A. nanus low-temperature stress resistance and that fascicular cambium cells could be involved in AnGolS1 mRNA transcription, galactinol transportation and coordination under low-temperature stress.

Indexed as

Cloning, MolecularAmino Acid SequenceCold TemperatureDroughtsFabaceaeGalactosyltransferasesIn Situ HybridizationPhylogenyPlant LeavesPlant ProteinsPlant RootsPlants, Genetically ModifiedPlant StemsReal-Time Polymerase Chain ReactionRecombinant Fusion ProteinsRNA, MessengerGalactosyltransferasesinositol 1-alpha-galactosyltransferasePlant ProteinsRecombinant Fusion ProteinsRNA, MessengerSalts

Identifiers

PMID27786294
PMCPMC5081558
OpenAlexW2544275470

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.