ArticleScientific reports2016
Genome-wide identification of endogenous RNA-directed DNA methylation loci associated with abundant 21-nucleotide siRNAs in Arabidopsis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
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- Homeobox 27, a Homeodomain Transcription Factor, Confers Tolerances to CMV by Associating with Cucumber Mosaic Virus 2b Protein.Pathogens (Basel, Switzerland) · 2022Article
- Cucumber RDR1s and cucumber mosaic virus suppressor protein 2b association directs host defence in cucumber plants.Molecular plant pathology · 2021Article
- Environmental RNAi pathways in the two-spotted spider mite.BMC genomics · 2021Article
- Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology.Frontiers in plant science · 2021Review
- Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis.Molecular plant pathology · 2019Article
- Roles of Small RNAs in Virus-Plant Interactions.Viruses · 2019Review
- CMV2b-Dependent Regulation of Host Defense Pathways in the Context of Viral Infection.Viruses · 2018Article
- Transposon-associated epigenetic silencing during Pleurotus ostreatus life cycle.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Epigenetic Changes in the Regulation ofViruses · 2018Article
- Phytopathogen-induced changes to plant methylomes.Plant cell reports · 2018Review
- Methods for discovering genomic loci exhibiting complex patterns of differential methylation.BMC bioinformatics · 2017Article
- Extensive transcriptomic and epigenomic remodelling occurs during Arabidopsis thaliana germination.Genome biology · 2017Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Arabidopsis, the 24-nucleotide (nt) small interfering RNAs (siRNAs) mediates RNA-directed DNA methylation (RdDM) and transcriptional gene silencing (TGS) of transposable elements (TEs). In the present study, we examined genome-wide changes in DNA methylation and siRNA accumulation in Arabidopsis induced by expression of the Cucumber mosaic virus silencing suppressor protein 2b known to directly bind to both the 21/24-nt siRNAs as well as their associated Argonaute proteins. We demonstrated a genome-wide reduction of CHH and CHG methylation in the 2b-transgenic plants. We found that 2b suppressed RdDM not only at the previously annotated loci directed by 24-nt siRNAs but also a new set of loci associated with 21/22-nt siRNAs. Further analysis showed that the reduced methylation of TEs and coding genes targeted by 21/22-nt siRNAs was associated with sequestration of the duplex siRNAs by the 2b protein but not with changes in either siRNA production or transcription. Notably, we detected both the deletion and/or the transposition of multicopy TEs associated with 2b-induced hypomethylation, suggesting potential TE reactivation. We propose that the silencing of many TEs in Arabidopsis is controlled by the 24- and 21-nt endogenous siRNAs analogous to Drosophila TE silencing by PIWI-interacting RNAs and siRNAs.
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