ArticleScientific reports2016
Synchronizing stochastic circadian oscillators in single cells of Neurospora crassa.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Origin of the clock inFrontiers in molecular biosciences · 2025Review
- The clock in growing hyphae and their synchronization in Neurospora crassa.Communications biology · 2024Article
- The macroscopic limit to synchronization of cellular clocks in single cells of Neurospora crassa.Scientific reports · 2022Article
- Synchronization between peripheral circadian clock and feeding-fasting cycles in microfluidic device sustains oscillatory pattern of transcriptome.Nature communications · 2021Article
- Circadian rhythm shows potential for mRNA efficiency and self-organized division of labor in multinucleate cells.PLoS computational biology · 2021Article
- Wild Isolates ofMicroorganisms · 2020Article
- Identifying a stochastic clock network with light entrainment for single cells of Neurospora crassa.Scientific reports · 2020Article
- What is Phase in Cellular Clocks?The Yale journal of biology and medicine · 2019Article
- Period-amplitude co-variation in biomolecular oscillators.IET systems biology · 2018Article
- Ensemble methods for stochastic networks with special reference to the biological clock of Neurospora crassa.PloS one · 2018Article
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Authors and funding
9 authors.
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Abstract
The synchronization of stochastic coupled oscillators is a central problem in physics and an emerging problem in biology, particularly in the context of circadian rhythms. Most measurements on the biological clock are made at the macroscopic level of millions of cells. Here measurements are made on the oscillators in single cells of the model fungal system, Neurospora crassa, with droplet microfluidics and the use of a fluorescent recorder hooked up to a promoter on a clock controlled gene-2 (ccg-2). The oscillators of individual cells are stochastic with a period near 21 hours (h), and using a stochastic clock network ensemble fitted by Markov Chain Monte Carlo implemented on general-purpose graphical processing units (or GPGPUs) we estimated that >94% of the variation in ccg-2 expression was stochastic (as opposed to experimental error). To overcome this stochasticity at the macroscopic level, cells must synchronize their oscillators. Using a classic measure of similarity in cell trajectories within droplets, the intraclass correlation (ICC), the synchronization surface ICC is measured on >25,000 cells as a function of the number of neighboring cells within a droplet and of time. The synchronization surface provides evidence that cells communicate, and synchronization varies with genotype.
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