ArticleMolecular microbiology2017
PrgU: a suppressor of sex pheromone toxicity in Enterococcus faecalis.
Article in Molecular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- Prolonged zinc exposure modulates biofilm metabolic activity and conjugation in Enterococcus faecalis.BMC microbiology · 2026Article
- Pheromone cCF10 Enhances Persister Formation inMicroorganisms · 2026Article
- A newly discoveredFrontiers in microbiology · 2026Article
- Insights into ecology, pathogenesis, and biofilm formation ofMicrobiology and molecular biology reviews : MMBR · 2025Review
- Vancomycin-variable enterococci in sheep and cattle isolates and whole-genome sequencing analysis of isolates harboringIranian journal of veterinary research · 2023Article
- Pheromone Activity after Stimulation with Ampicillin in a Plasmid-FreeMicroorganisms · 2022Article
- Proteomic Characterization of Virulence Factors and Related Proteins inInternational journal of molecular sciences · 2022Article
- Dynamics of plasmid-mediated niche invasion, immunity to invasion, and pheromone-inducible conjugation in the murine gastrointestinal tract.Nature communications · 2022Article
- Enterococcal PrgU Provides Additional Regulation of Pheromone-Inducible Conjugative Plasmids.mSphere · 2021Article
- Article
- Ratiometric quorum sensing governs the trade-off between bacterial vertical and horizontal antibiotic resistance propagation.PLoS biology · 2020Article
- Competent but complex communication: The phenomena of pheromone-responsive plasmids.PLoS pathogens · 2020Review
- Single-Cell Analysis Reveals that the Enterococcal Sex Pheromone Response Results in Expression of Full-Length Conjugation Operon Transcripts in All Induced Cells.Journal of bacteriology · 2020Article
- Gene Duplications in the Genomes of Staphylococci and Enterococci.Frontiers in molecular biosciences · 2020Article
- Enterococcal Genetics.Microbiology spectrum · 2019Review
- Regulation of Gram-Positive Conjugation.Frontiers in microbiology · 2019Review
- TraN: A novel repressor of an Enterococcus conjugative type IV secretion system.Nucleic acids research · 2018Article
- PrgB promotes aggregation, biofilm formation, and conjugation through DNA binding and compaction.Molecular microbiology · 2018Article
- Mechanistic Features of the Enterococcal pCF10 Sex Pheromone Response and the Biology of Enterococcus faecalis in Its Natural Habitat.Journal of bacteriology · 2018Review
- Type IV secretion in Gram-negative and Gram-positive bacteria.Molecular microbiology · 2018Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Upon sensing of the peptide pheromone cCF10, Enterococcus faecalis cells carrying pCF10 produce three surface adhesins (PrgA, PrgB or Aggregation Substance, PrgC) and the Prg/Pcf type IV secretion system and, in turn, conjugatively transfer the plasmid at high frequencies to recipient cells. Here, we report that cCF10 induction is highly toxic to cells sustaining a deletion of prgU, a small orf located immediately downstream of prgB on pCF10. Upon pheromone exposure, these cells overproduce the Prg adhesins and display impaired envelope integrity, as evidenced by antibiotic susceptibility, misplaced division septa and cell lysis. Compensatory mutations in regulatory loci controlling expression of pCF10-encoded prg/pcf genes, or constitutive PrgU overproduction, block production of the Prg adhesins and render cells insensitive to pheromone. Cells engineered to overproduce PrgB, even independently of other pCF10-encoded proteins, have severely compromised cell envelopes and strong growth defects. PrgU has an RNA-binding fold, and prgB-prgU gene pairs are widely distributed among E. faecalis isolates and other enterococcal and staphylococcal species. Together, our findings support a model in which PrgU proteins represent a novel class of RNA-binding regulators that act to mitigate toxicity accompanying overproduction of PrgB-like adhesins in E. faecalis and other clinically-important Gram-positive species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.