ArticleInternational journal of legal medicine2017
A preliminary assessment of the ForenSeq™ FGx System: next generation sequencing of an STR and SNP multiplex.
Article in International journal of legal medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Development and validation of a STR and SNP multiplex detection system (88 STRs and 348 SNPs) using massively parallel sequencing.International journal of legal medicine · 2026Article
- Forensic DNA phenotyping: a review on SNP panels, genotyping techniques, and prediction models.Forensic sciences research · 2025Review
- Population genetic analyses of Eastern Chinese Han nationality using ForenSeq™ DNA Signature Prep Kit.Molecular genetics and genomics : MGG · 2024Article
- Forensic analysis and sequence variation of 133 STRs in the Hakka population.Frontiers in genetics · 2024Article
- Validation and beyond: Next generation sequencing of forensic casework samples including challenging tissue samples from altered human corpses using the MiSeq FGx system.Journal of forensic sciences · 2022Article
- Exploring STR sequencing for forensic DNA intelligence databasing using the Austrian National DNA Database as an example.International journal of legal medicine · 2021Article
- Comprehensive Insights Into Forensic Features and Genetic Background of Chinese Northwest Hui Group Using Six Distinct Categories of 231 Molecular Markers.Frontiers in genetics · 2021Article
- Identification of sequence polymorphisms at 58 STRs and 94 iiSNPs in a Tibetan population using massively parallel sequencing.Scientific reports · 2020Article
- Massive parallel sequencing in forensics: advantages, issues, technicalities, and prospects.International journal of legal medicine · 2020Review
- Inter-laboratory study on standardized MPS libraries: evaluation of performance, concordance, and sensitivity using mixtures and degraded DNA.International journal of legal medicine · 2020Article
- Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems.International journal of legal medicine · 2019Article
- Massively parallel sequencing analysis of nondegraded and degraded DNA mixtures using the ForenSeq™ system in combination with EuroForMix software.International journal of legal medicine · 2019Article
- Massively parallel sequencing techniques for forensics: A review.Electrophoresis · 2018Review
- Comparison between magnetic bead and qPCR library normalisation methods for forensic MPS genotyping.International journal of legal medicine · 2018Article
- Sequencing of 231 forensic genetic markers using the MiSeq FGx™ forensic genomics system - an evaluation of the assay and software.Forensic sciences research · 2018Article
- Investigation of the STR loci noise distributions of PowerSeq™ Auto System.Croatian medical journal · 2017Article
- Qualitative and quantitative assessment of Illumina's forensic STR and SNP kits on MiSeq FGx™.PloS one · 2017Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ForenSeq™ FGx System (Illumina, San Diego, CA) was initially evaluated in concordance with SWGDAM guidelines for internal validation to determine the quality of the system's components: the ForenSeq™ DNA Signature Prep Kit reagents, the MiSeq FGx™ instrument, and the ForenSeq™ Universal Analysis Software, for the analysis of targeted, forensically informative single nucleotide polymorphisms (SNPs) and short tandem repeats (STRs). This multiplex consisted of STRs (autosomal, X, and Y) and SNPs (identity, ancestry, and phenotypic) that were run using one preparation process. Overall, the ForenSeq™ FGx System performed as well as the traditional capillary electrophoresis-based method in producing usable profile information, along with additional information that could aid in investigative leads. The MiSeq FGx™ System was validated using DNA samples in studies testing reproducibility, repeatability, concordance, sensitivity, and mock case single donor samples. Overall, genotyping results for STRs and SNPs were concordant with the profiles generated from conventional STR analysis using Identifiler and SNPs typed by 23andMe analysis. Genotypes of the ForenSeq™ aSNPs were used to evaluate biogeographical ancestry estimations using ForenSeq™ Universal Analysis Software, FROG-kb database (KIDD aiSNP 55 panel), and 23andMe. The system was shown to provide reproducible genotypes and reliable results were obtained at levels as low as 50 pg. All mock case samples were concordant with the donor profile. The results support consideration of the ForenSeq™ FGx System as an acceptable alternative to current STR and SNP analysis, pending formal developmental and internal validation studies.
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